Protein Info for RPSI07_RS07320 in Ralstonia solanacearum PSI07

Annotation: phosphogluconate dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01196: phosphogluconate dehydratase
  • amino acids 7 to 603 (597 residues), 1016.1 bits, E=0
• PF00920: ILVD_EDD
  • amino acids 67 to 600 (534 residues), 559.6 bits, E=3.5e-172

Best Hits

Swiss-Prot: 66% identical to EDD_PSEAE: Phosphogluconate dehydratase (edd) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01690, phosphogluconate dehydratase [EC: 4.2.1.12] (inferred from 100% identity to rsl:RPSI07_mp1664)

MetaCyc: 61% identical to phosphogluconate dehydratase (Escherichia coli K-12 substr. MG1655)
Phosphogluconate dehydratase. [EC: 4.2.1.12]

Predicted SEED Role

"Phosphogluconate dehydratase (EC 4.2.1.12)" in subsystem Entner-Doudoroff Pathway (EC 4.2.1.12)

MetaCyc Pathways

• superpathway of glycolysis and the Entner-Doudoroff pathway (15/17 steps found)
• Entner-Doudoroff pathway I (9/9 steps found)
• Entner-Doudoroff shunt (2/2 steps found)

KEGG Metabolic Maps

• Pentose phosphate pathway

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.12

Protein Sequence (609 amino acids)

>RPSI07_RS07320 phosphogluconate dehydratase (Ralstonia solanacearum PSI07)
MALHRVLGDVTQRIIDRSRGPRQAYLETTRKNAGRKVERSLLSCTNLAHGFAAMEGEAKI
RLKALERPNIGIVSAYNDMLSAHQPLEVFPAWIKEAALEAGGTAQFAGGVPAMCDGVTQG
QDGMELSLFSRDTIALSTAVALSHQMFDAALYLGVCDKIVPGLVIGALSFGHLPAVFVPG
GPMTTGMSNDDKARTRQLYAEGKLSRADLLEAESKSYHGAGTCTFYGTANSNQMLMEIMG
LHLPGTAFINPNTPLREALTRESARQVLKLVHGGGQYTPIADVLDERAFVNGIVGLLATG
GSTNHTLHLIAMARAAGILLTWDDFDDLSSVIPLLARVYPNGKADVNEFQAAGGLSIVIR
ELLDAGLLHDNVTTVMGKGLRQHSREPFLQDGKLVWRDGAAASLDTNIVRGVAEPFSPDG
GLRVLDGNLGRSVIKVSAVKPEHRFVEAPARVFHAQDDLIHAFKAGELERDFVAVLPYQG
PAANGMPELHKLTPTLSVLQERGFKVALVTDGRMSGASGKVPAAIHVTPEALTGGPLARV
RDGDIVVLDAEAGTLSVRVPEAEWNARKVTTPDLAHYHAGVGRDLFSGFRRNVSAAETGA
CTLFVSAGA