Protein Info for RPSI07_RS05820 in Ralstonia solanacearum PSI07

Annotation: L-aspartate oxidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 536 transmembrane" amino acids 15 to 33 (19 residues), see Phobius details TIGR00551: L-aspartate oxidase" amino acids 14 to 502 (489 residues), 509.2 bits, see alignment E=5.7e-157 PF00890: FAD_binding_2" amino acids 16 to 386 (371 residues), 281.4 bits, see alignment E=1.5e-87 PF02910: Succ_DH_flav_C" amino acids 429 to 507 (79 residues), 47.4 bits, see alignment E=1.8e-16

Best Hits

Swiss-Prot: 95% identical to NADB2_RALSO: L-aspartate oxidase 2 (nadB2) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K00278, L-aspartate oxidase [EC: 1.4.3.16] (inferred from 100% identity to rsl:RPSI07_mp1299)

Predicted SEED Role

"L-aspartate oxidase (EC 1.4.3.16)" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 1.4.3.16)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.4.3.16

Use Curated BLAST to search for 1.4.3.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (536 amino acids)

>RPSI07_RS05820 L-aspartate oxidase (Ralstonia solanacearum PSI07)
MTSGQSKHEIRDMKFDVLVVGEGLAALTLLLHLPPSLKVGVISRNKYDEPSSYWAQGGIS
AVFSVDDDHDKHIRDTLLAGDGLCDAAAVRQIVREGGDVLRWLIDQDVPFTREDGEIHLT
REGGHSERRVAHVDDMTGRGIMRALQAKVARLPNVIWIRQYEVVELISDGQAVSGVIAES
LADGEVSVFSAPTVVLATGGLTGLYQYATNPHASKGEAIAMAWRAGATIENLEFVQFHPT
AFQVEGRVISLITEAVRGEGGLLYNVANERFMPGYSSQQELAPRDVVARAIYSEMQAHGT
SHVWLDITHQGAAFVEQHFPNLVEITRAHGCDLSQHRVPVSPAAHYTCGGISADVAGRTN
VDGLYAIGEVANCGLHGANRLASNSLLECVVMGQACAQAVAGTAGSTSRLRLTLPERIET
PFHPNLLADLRAILWNHAGIVRSNTGLQIGLQNMAQLRERHASVLPYGQALRAQNIFDAA
HLVLLSASARKESRGGHFNKDHADKAEAHATRIPGVPLNFWATGENAPHAAQLAAA