Protein Info for RPSI07_RS05610 in Ralstonia solanacearum PSI07

Annotation: nitrite reductase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 851 PF07992: Pyr_redox_2" amino acids 3 to 284 (282 residues), 181.4 bits, see alignment E=8e-57 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 5 to 798 (794 residues), 1146.6 bits, see alignment E=0 PF00070: Pyr_redox" amino acids 147 to 223 (77 residues), 55.7 bits, see alignment E=1.8e-18 PF18267: Rubredoxin_C" amino acids 319 to 388 (70 residues), 42.9 bits, see alignment E=1.1e-14 PF04324: Fer2_BFD" amino acids 423 to 470 (48 residues), 38.2 bits, see alignment 4.4e-13 PF03460: NIR_SIR_ferr" amino acids 558 to 621 (64 residues), 47.1 bits, see alignment 5e-16 PF01077: NIR_SIR" amino acids 631 to 752 (122 residues), 71.7 bits, see alignment E=1.5e-23

Best Hits

Swiss-Prot: 62% identical to NIRB_ECOLI: Nitrite reductase (NADH) large subunit (nirB) from Escherichia coli (strain K12)

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 82% identity to bph:Bphy_5659)

MetaCyc: 62% identical to nitrite reductase (NADH) large subunit (Escherichia coli K-12 substr. MG1655)
RXN-13854 [EC: 1.7.1.15]

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.15 or 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (851 amino acids)

>RPSI07_RS05610 nitrite reductase large subunit (Ralstonia solanacearum PSI07)
MTMKIVVIGHGMVGHKFLESLLHAPGHDLHVTVLCEEPRPAYDRVHLSEFFSGKTAEDLS
LVAPGFFDRDDVVLRLNARAVSIDLAAKQVTASTGDVLAYDKLVMATGSSPFVPSIPGKD
RADCFVYRTIEDLEAMLECGQRAKTGVVIGGGLLGLECAKALRDMRLQTHVVEFAGRLMA
MQVDDGGGRMLRRKIEDLGVTVHTQKNTVEIVDGEAGTHRMQFADGTHLDADMIVFSAGI
RPRDELARQCGLAVGERGGIVIDSECRTSDPDVYAIGECALWGGKVYGLVAPGYEMARIT
AKQILAAEEATPFNGADMSTKLKLMGVDVASLGDAQGSTPGSRSVQFTDERKQIYKKLVV
SEDGKYLLGGVLVGDASEYGTLLQMMLNRIELPEAPEFLILPQADGNARPALGVDALPDS
AQICSCNDVSKGALCQAVCAGAATVGALKDATRAGTSCGGCVPLMTQVMKAEMKKQGLAV
NNHLCEHFPYSRQELYHLVRVGRFQTFEALLEAHGHGMGCDICKPVVASVLASCWNDFVL
KKEHAGLQDSNDYFLANIQRDGTYSVVPRMPGGEVTPEGLIAVGQVAKKYGLYTKITGGQ
RVDLFGARLEQLPLIWEELIAAGFESGHAYGKSLRTVKSCVGSTWCRYGVGDSVGFAIRL
ENRYKGLRSPHKLKFGVSGCTRECAEAQGKDVGVIATEKGWNLYVCGNGGMKPRHAELLA
ADLDEATLVKLVDRFLMFYVRTADRLQRTSTWRDSLEGGLDYLKDVIVGDKLGIAAELEA
EMQHVVDTYQDEWKTAVTNPEVRKRFRHFVNSDAADANVVFVEERGQIRPATPEERHNTR
PVRIPVVAEAA