Protein Info for RPSI07_RS04120 in Ralstonia solanacearum PSI07

Annotation: arsenate reductase (azurin) large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 828 TIGR02693: arsenite oxidase, large subunit" amino acids 7 to 808 (802 residues), 1289.9 bits, see alignment E=0 PF18465: Rieske_3" amino acids 22 to 114 (93 residues), 118.8 bits, see alignment E=1.6e-38 PF00384: Molybdopterin" amino acids 120 to 574 (455 residues), 88.8 bits, see alignment E=5.6e-29 PF01568: Molydop_binding" amino acids 697 to 799 (103 residues), 57.4 bits, see alignment E=2.1e-19

Best Hits

Swiss-Prot: 71% identical to AIOA_ALCFA: Arsenite oxidase subunit AioA (aioA) from Alcaligenes faecalis

KEGG orthology group: K08356, arsenite oxidase large subunit [EC: 1.20.98.1] (inferred from 100% identity to rsl:RPSI07_mp0920)

MetaCyc: 71% identical to arsenite oxidoreductase large subunit (Alcaligenes faecalis)
1.20.98.1-RXN [EC: 1.20.9.1]; RXN-12586 [EC: 1.20.9.1, 1.20.2.1]

Predicted SEED Role

"Arsenate reductase (Azurin) (EC 1.20.98.1)" (EC 1.20.98.1)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 1.20.9.1, 1.20.98.1

Use Curated BLAST to search for 1.20.2.1 or 1.20.9.1 or 1.20.98.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (828 amino acids)

>RPSI07_RS04120 arsenate reductase (azurin) large subunit (Ralstonia solanacearum PSI07)
MATNKDRVALPPVSAQKTNLTCHFCIVGCGYHVFKWPEDTEGGRAPSQNALGLDFRKQLP
PLSVVMTPNMQNTITDKDGRRYSILIVPDKQCAVNRGLSSTRGGQLAKVMYNPDGVAKER
LRSPRVYVADQWVDTSWDDALALYAGLTKRILDRDGPAGLAFDCFDHGGAGGGFENTWGT
GKLMFTALKTPLVRIHNRPAYNSECHATREMGIGELNNSYEDAELADVIVAIGCNSYETQ
TNYFLAHWLPNLQGQTVDKRKQRFPGESPVAAKVIFVDPRRTTTIAVAEQAAGKANVLHL
DIEPGTDIALFDGLLTYVVDQGWHDKEFIAAYTKGFDQALRANRLSLEEASRITGVPVDK
LRQAAEWAYKPNAAGQRPRTMHAYEKGIIWGNDNYLIQSALVDLVLATHNVGRRGTGVVR
MGGHQEGYARPPYPGNSKIYVDQEIIHGKGLMYTAWGANPFQTTLNAQEHRAVILHRANI
VKEAMVQVRGAPAAQMIDAIYDAVRNKGGLFVTNINLYPTKLAEAAHLMLPAAHPGEMNL
TSMNGERRMRLSERFMDPPGTARPDCLIAADIANALKRMYEAEGKREMATRFSGFDWKTE
EDAFNDGFRQAGQPGAGHIDSQGGDTGNLVTFERLHAMGNNGVQLPVKEYRDGKLVGTEM
LYTDGKFDTTDGKAEFKPATWPGLPKPVADQKAKYRFWINNGRVNEVWQTLYHDQYNEFV
RGRVPMAFLEINPEDARSLGIGSGDVVEVYNDYGSTYAMAYLEPDIKRNQTFMQFGHFNG
VMGNVTTPWTDRNVVPYYKGTWANLRRVGTVQDFKETVSFKKRRFESA