Protein Info for RPSI07_RS03935 in Ralstonia solanacearum PSI07

Annotation: proline dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 523 PF02543: Carbam_trans_N" amino acids 103 to 312 (210 residues), 70 bits, see alignment E=2.7e-23 PF16861: Carbam_trans_C" amino acids 355 to 515 (161 residues), 190.6 bits, see alignment E=1.7e-60

Best Hits

KEGG orthology group: K04128, hydroxymethyl cephem carbamoyltransferase [EC: 2.1.3.7] (inferred from 100% identity to rsl:RPSI07_mp0876)

Predicted SEED Role

"macromolecule metabolism; macromolecule synthesis, modification"

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.3.7

Use Curated BLAST to search for 2.1.3.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (523 amino acids)

>RPSI07_RS03935 proline dehydrogenase (Ralstonia solanacearum PSI07)
MKIFAMKPGHDGAVALVDADAGKLVWSLEAEKDSFPRFEAFNPAQMVDAAERLDVMPDVF
AVSGWGKGSMAANAPIGAGYYGWDVNAVQQRAARMFGHEVKYFSSSHERSHLWCSYGLSP
FEQGQPCYVLVWEGALGDFYQIDEKLNVTSLGRVMMTPGNKYSFLYALADPSFTLPKGKL
RNEDPGKLMALCAYGESGEMDDDERELTEFLLKRDSILATLSKDDMRWTKFYNIGLDHPA
FTRLAKRYSDEIFNRFYLYAKEHLTQGYPLLISGGCGLNCDWNTMWRNAGLFRDVFVPPC
TNDTGSAIGTAIDAMREFTGKAKVEWNVYADRPFVDDTPHMPDVDVYPLDLKHVASFLSE
QKVVAWTQGRCEIGPRALGNRSILAAPFTDAMRDRLNHIKKREGFRPVAPICLEEDVSEH
FDWQGPSPHMLYFQQVNNHDLRAITHVDGTARTQTVNREQNPNMHSLLSAFKQLTGAGVL
CNTSLNFNGTGFINRTSDLYQYCKSTGIEGFVYDDKFCVVRGA