Protein Info for RPSI07_RS03480 in Ralstonia solanacearum PSI07
Annotation: glucarate dehydratase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01706, glucarate dehydratase [EC: 4.2.1.40] (inferred from 100% identity to rsl:RPSI07_mp0773)Predicted SEED Role
"Glucarate dehydratase (EC 4.2.1.40)" in subsystem D-galactarate, D-glucarate and D-glycerate catabolism (EC 4.2.1.40)
MetaCyc Pathways
- D-glucarate degradation II (3/3 steps found)
- superpathway of D-glucarate and D-galactarate degradation (3/5 steps found)
- D-glucarate degradation I (2/4 steps found)
- superpathway of microbial D-galacturonate and D-glucuronate degradation (13/31 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.40
Use Curated BLAST to search for 4.2.1.40
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (469 amino acids)
>RPSI07_RS03480 glucarate dehydratase (Ralstonia solanacearum PSI07) MTLQNIETGARAQTPRVTQMQVIPVAGRDSMLLNLCGAHAPFFTRNLVILTDSAGRTGVG EVPGGEGIRRALERIAPLVAGQPIGRANGVLNGIRRALAGNGHAAHQATVHEVTSASEAA VLRQPHEINLRMDNVITAVEAALLDLLGQHLEVPVAELLGAGQQRDAVPMLAYLFYVGDR CKTDLPYLDGAGTADGWLRLRHEAAMTPAQIARLAEAAAERYGFADFKLKGGVMRGAEEM EAIAAIKARFPQARVTLDPNGAWPLAEAIALCKGQGHLLAYAEDPCGPEAGYSGREIMAE FKRATGVPTATNMIATDWRQLGHAVPLQAVDIPLADPHFWTMQGSVRVAQLCDDWGLTWG SHSNNHFDVSLAMFTHVAAAAPGNITAIDTHWIWQEGQERLTREPLRIEGGQVRVPERPG LGIELDMERVMQAHALYQTLGTGARDDAMAMQYLVPGWTYDPKRPSLAH