Protein Info for RPSI07_RS02235 in Ralstonia solanacearum PSI07

Annotation: membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06180: CbiK
  • amino acids 11 to 93 (83 residues), 27.6 bits, E=2.7e-10
• PF01903: CbiX
  • amino acids 20 to 124 (105 residues), 92.7 bits, E=2.6e-30
  • amino acids 150 to 253 (104 residues), 94.3 bits, E=8e-31
• PF02570: CbiC
  • amino acids 320 to 514 (195 residues), 220.9 bits, E=2e-69

Best Hits

KEGG orthology group: K06042, precorrin-8X methylmutase [EC: 5.4.1.2] (inferred from 100% identity to rsl:RPSI07_mp0507)

Predicted SEED Role

"Sirohydrochlorin cobaltochelatase (EC 4.99.1.3) / Cobalt-precorrin-8x methylmutase (EC 5.4.1.2)" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis or Experimental tye (EC 4.99.1.3, EC 5.4.1.2)

MetaCyc Pathways

• adenosylcobalamin biosynthesis II (aerobic) (29/33 steps found)
• adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
• cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (13/15 steps found)
• cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) (11/13 steps found)
• siroheme biosynthesis (3/4 steps found)
• factor 430 biosynthesis (3/7 steps found)

KEGG Metabolic Maps

• Porphyrin and chlorophyll metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.99.1.3

Use Curated BLAST to search for 4.99.1.3 or 5.4.1.2

Protein Sequence (531 amino acids)

>RPSI07_RS02235 membrane protein (Ralstonia solanacearum PSI07)
MKTADDSTHCADYAIVLAGHGSRDPDGVDEFMALVALLRERAGERTVAHGFLEFATPTID
EAVDAVVAAGAKTVVMVPGVLLAATHAKNDMPSELLALKQAHPEVDFRFGAAMDLHPKLL
ALCRERIVEAEGRSAQTVSRADTCLVVVGRGTSDPDANSEIAKLARMLEEGMGFGTSFVC
YAGTARPLVADGLKAAALLGYRRIVVLPYFLFDGVLVKRIYSAADDLAARHPEIEMLRAP
YLGVHPHVADVFLERAREGIEGRAAMNCALCKYRVQIVGFEEQVGTPQQGHHGLVRGLLG
RNGTPPAPATDWPPYVPHPIEAESMRIIDAGRDWSGVPADHQIVLKRLVHTSGDFDIVDD
LYFSPGAVETGIRALLRCRRIVADVTMVQTGLKRAVLDQLSVETWCGVHDEETRLLAEAH
GITRSAAGIRRAWQKFGNDVVVAIGDAPTAILELVRLVREHGWRPQLVVGLPVGFVGTRE
SKEALRRLMQVPRITNRGTRGGSPWAASAVNALMIAAIDQVYRQNQTQQQA