Protein Info for RPSI07_RS01430 in Ralstonia solanacearum PSI07
Annotation: flagellar assembly peptidoglycan hydrolase FlgJ
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K02395, flagellar protein FlgJ (inferred from 100% identity to rsl:RPSI07_mp0324)Predicted SEED Role
"Flagellar protein FlgJ [peptidoglycan hydrolase] (EC 3.2.1.-)" in subsystem Flagellum (EC 3.2.1.-)
KEGG Metabolic Maps
- Ascorbate and aldarate metabolism
- Glycosaminoglycan degradation
- Nucleotide sugars metabolism
- Ubiquinone and menaquinone biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 3.2.1.-
Use Curated BLAST to search for 3.2.1.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (362 amino acids)
>RPSI07_RS01430 flagellar assembly peptidoglycan hydrolase FlgJ (Ralstonia solanacearum PSI07) MNRTDISNRLALDAQGFESLKQTARTDPTGAAKTVAKQFDAIFVNMMLKQMREASPQSGL FDSASSKMYTSMFDQQLSQTIASRGVGVADQLLKQMLRQAAKTNPDASGPVNTALSRTTA TNTALPGAAGSTATDAAKAIARASLNGLAASAAAGTEDDGSGISTVPRPGLDERVQRALA ALRKQAEAEAQSGGTLPEADLSSVASQPAGDRMSSFYSKLIDHATQASQDTGIPANFMIG HAALESGWGRREIRAKDGSNTHNLFGIKAGGSWTGPTAEVTTTEYVGGVAHKVKEKFRAY GSYAEAFKDYANLLSSNPRYSNVVAAGAGKDAASFARGLQRAGYATDPNYANKIMAVLRQ IA