Protein Info for RPSI07_RS00595 in Ralstonia solanacearum PSI07

Annotation: aconitate hydratase AcnA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01341: aconitate hydratase 1
  • amino acids 26 to 868 (843 residues), 961.3 bits, E=1.9e-293
• PF00330: Aconitase
  • amino acids 72 to 543 (472 residues), 474.9 bits, E=3.2e-146
• PF00694: Aconitase_C
  • amino acids 670 to 796 (127 residues), 115.1 bits, E=2.9e-37

Best Hits

KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 100% identity to rsl:RPSI07_mp0134)

Predicted SEED Role

"Aconitate hydratase (EC 4.2.1.3)" in subsystem Propionate-CoA to Succinate Module or Serine-glyoxylate cycle or TCA Cycle (EC 4.2.1.3)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (24/26 steps found)
• superpathway of glyoxylate bypass and TCA (12/12 steps found)
• TCA cycle I (prokaryotic) (10/10 steps found)
• partial TCA cycle (obligate autotrophs) (8/8 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• glyoxylate cycle (6/6 steps found)
• TCA cycle II (plants and fungi) (8/9 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle III (animals) (8/10 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• TCA cycle VII (acetate-producers) (7/9 steps found)
• mixed acid fermentation (12/16 steps found)
• nitrogen remobilization from senescing leaves (6/8 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• reductive TCA cycle I (6/11 steps found)
• reductive TCA cycle II (6/12 steps found)
• methylaspartate cycle (11/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3

Use Curated BLAST to search for 4.2.1.3

Protein Sequence (891 amino acids)

>RPSI07_RS00595 aconitate hydratase AcnA (Ralstonia solanacearum PSI07)
MGNTYTPPQAVVSVGNVAYRVIDLPAMFGKTLERLPVVLRLLLENTLRNTEGRERQRAVE
GLLRWLEVGASEEEIAFQPARVLMHDTTSTPALVDIAAMRDALAEAGADPSVLNPVLPVD
VSVDHSLAVEYSARADAAVLNLQTEMRRNAERYRFLRWASKALRGVRIHPPGTGIMHTIN
LEQLATVASVQTLDGVPWLVPDTLIGTDSHTPMINGVGVLGWGVGGLEAQTVMFGMPVMQ
RIPDVIGVRLLGALPTGTSATDLALTVTKALRDIGVSGEFVEFFGPGVATLSAGERAVVA
NMAPEYGATTGFFPVDAQTLAYLRATGRPQAHVARVEALHRRAGLWFDPGNTPRYTRVVE
IDLAAVRLHVAGPRRPQDLLPYTDTGHALSAFDRTDAEAADGLPGHPVAIAAITSCTNTS
DPAQLIAAGLVARRARERGLRVPDWVKTSLAPGSPAAAEYLRRAGLMDDLSAVGFDIVGF
GCTTCIGNSGPLTAPIQQAIADGTAAPVAVLSGNRNFPGRVHPDLSLGFIMSPPLVIAFA
LAGDAARDLSRQPLQTAPDGTPVHLRDLWPTRAEIDTLVRRGTSPADYRHAFAIATANPA
WAAIDAPDAPRYPWDAGSTALRRPPFASADAGSQLGDYEAHPLLVLGDDITTDHISPASV
IPKDSFVADFLVARGDDRNDLNVFASRRGNWEVMVRAAFYSKTLVNLLAPGLPVAHTLHV
PSGQAMPVFEAAQRYRDDGASVVVLAGARYGTGSSRDWAAKGQRLLGVRAVIAVSFERIH
RSNLIGMGILPLRFPEGVSPRTLAIRPGDTIQVAARPERIGPRCAVPVRVRRADGSVETF
IATAAVETQLETALLRGGGVIPAILDKTLQTQRAAAQAEPQQAASPHSPMP