Protein Info for QEN71_RS42400 in Paraburkholderia sabiae LMG 24235

Annotation: nitrogenase molybdenum-iron protein subunit beta

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 519 PF11844: DUF3364" amino acids 1 to 56 (56 residues), 89.6 bits, see alignment 1.2e-29 TIGR01286: nitrogenase molybdenum-iron protein beta chain" amino acids 3 to 519 (517 residues), 809.3 bits, see alignment E=6.1e-248 PF00148: Oxidored_nitro" amino acids 70 to 491 (422 residues), 406.6 bits, see alignment E=1e-125

Best Hits

Swiss-Prot: 67% identical to NIFK_BRADU: Nitrogenase molybdenum-iron protein beta chain (nifK) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K02591, nitrogenase molybdenum-iron protein beta chain [EC: 1.18.6.1] (inferred from 99% identity to bph:Bphy_7755)

Predicted SEED Role

"Nitrogenase (molybdenum-iron) beta chain (EC 1.18.6.1)" in subsystem Nitrogen fixation (EC 1.18.6.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.6.1

Use Curated BLAST to search for 1.18.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (519 amino acids)

>QEN71_RS42400 nitrogenase molybdenum-iron protein subunit beta (Paraburkholderia sabiae LMG 24235)
MPQSTDKILDHELLFREPEYQELLRNKRENFEFNHPDEAVTQVAEWTKTEDYKQKNFARD
SLTVNPAKACQPLGAVFVANGFYKTLPFVHGSQGCVAYYRSHFSRHFKEPTSCVSSSMTE
DAAVFGGLNNMIDGLANAYNLYKPEMIAVSTTCMAEVIGDDLDAFIKNSKQKGSVPQDYD
VPFAHTPAFVGSHVTGYDNALLGILKYFWDGKAGTTTPLTRVTDDTVNFIGGFDGFVVGN
MKEIRRIFDLFGVKVNIICDPSETWNTPTDGEFRMYQGGTTKEEVERALNAKATFIFQEF
CCEKTAKFIADHGQEVISLNAPVGVGGTDHFLMEISRVTGRPIPAELEKERGQLVDAMAD
SQANLHGKRYALYGDPDQMLGYTQFLLELGAEPVHVLATNGNDGWAEKVRALLDASPYGA
GCKVYPKRDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLVRLVFPIFDRHHYHRYPT
WGYSGGLRILLALLDEFFETMDANTIDISKTDYSFDIVR