Protein Info for QEN71_RS42390 in Paraburkholderia sabiae LMG 24235

Annotation: nitrogenase iron protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 293 TIGR01287: nitrogenase iron protein" amino acids 5 to 277 (273 residues), 448.5 bits, see alignment E=4.1e-139 PF00142: Fer4_NifH" amino acids 5 to 275 (271 residues), 427.9 bits, see alignment E=3.5e-132 PF02374: ArsA_ATPase" amino acids 5 to 46 (42 residues), 29.5 bits, see alignment 9.1e-11 PF13614: AAA_31" amino acids 7 to 74 (68 residues), 35.3 bits, see alignment E=2.3e-12 PF01656: CbiA" amino acids 9 to 228 (220 residues), 40.2 bits, see alignment E=6.2e-14

Best Hits

Swiss-Prot: 88% identical to NIFH_ACIF5: Nitrogenase iron protein (nifH) from Acidithiobacillus ferrooxidans (strain ATCC 53993)

KEGG orthology group: K02588, nitrogenase iron protein NifH [EC: 1.18.6.1] (inferred from 98% identity to bge:BC1002_6929)

MetaCyc: 75% identical to [FeMo]-nitrogenase complex nitrogenase reductase component monomer (Klebsiella pneumoniae)

Predicted SEED Role

"Nitrogenase (molybdenum-iron) reductase and maturation protein NifH" in subsystem Nitrogen fixation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.6.1

Use Curated BLAST to search for 1.18.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (293 amino acids)

>QEN71_RS42390 nitrogenase iron protein (Paraburkholderia sabiae LMG 24235)
MSQLRQIAFYGKGGIGKSTTSQNTLAALSDLGQKILIVGCDPKADSTRLILHAKAQDTIL
SLAAEAGSVEDLELDDVMKIGYKDIRCVESGGPEPGVGCAGRGVITSINFLEENGAYDGV
DYVSYDVLGDVVCGGFAMPIRENKAQEIYIVMSGEMMAMYAANNISKGILKYANSGGVRL
GGLICNERKTDKELELAESLATMLGTKLIHFVPRDNIVQHAELRRMTVIEYAPDSSQAGQ
YRALAEKVHHNGGNGVVPTPITMDQLEDLLMEKGIMVQVDESQVGKTAAELTA