Protein Info for QEN71_RS42220 in Paraburkholderia sabiae LMG 24235

Annotation: FAD-dependent monooxygenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 436 PF01494: FAD_binding_3" amino acids 8 to 193 (186 residues), 35 bits, see alignment E=4e-12 PF01134: GIDA" amino acids 8 to 165 (158 residues), 30 bits, see alignment E=1.2e-10 PF12831: FAD_oxidored" amino acids 8 to 164 (157 residues), 31.1 bits, see alignment E=6.7e-11 PF13450: NAD_binding_8" amino acids 11 to 42 (32 residues), 22 bits, see alignment (E = 6.3e-08)

Best Hits

Swiss-Prot: 66% identical to FIXC_AZOVI: Protein FixC (fixC) from Azotobacter vinelandii

KEGG orthology group: K00313, electron transfer flavoprotein-quinone oxidoreductase [EC: 1.5.5.-] (inferred from 98% identity to bph:Bphy_7737)

MetaCyc: 66% identical to quinone reductase (NADH,flavodoxin) complex electron transfer catalytic subunit (Azotobacter vinelandii)
1.19.1.M1 [EC: 1.19.1.M1]

Predicted SEED Role

"Probable electron transfer flavoprotein-quinone oxidoreductase FixC (EC 1.5.5.-)" in subsystem Acetyl-CoA fermentation to Butyrate (EC 1.5.5.-)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.19.1.M1 or 1.5.5.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (436 amino acids)

>QEN71_RS42220 FAD-dependent monooxygenase (Paraburkholderia sabiae LMG 24235)
MKNTSRFDAIVVGAGPSGNAAAYVMAKGGLEVLQIERGEYPGSKNVQGAILYANALEQII
PDFRDHAPLERHIIEQRMWMLDDTSFVGTHVRSDDYNKPPYNRYTIIRAQFDKWFSSKVR
EAGALLICETTVNHLIMDGDQVVGVQCDREQGEVYADIVILADGVNSTLARKAGFHGEIE
ASNVALAVKEILFMPEETIRQRFNIGDEEGVVIEMVGRITEGMAGTGFLYTNKESLTIGV
GCMLSDFKKNSNRVSPYVLLERMKRHPSIVPLIVGGEMKEYCAHLIPEGGFHAIPQLYGN
GWMIVGDSGGFVNAAHREGCNLAMTTGRLAAETAIAAKAVGRGYRANALATYKDALDDSF
VMKDLYRYRDMPKILHRNPQFFTTYPDLVAKAARTMMTVDGIDKKTKKHEVMSSFRESRS
LTGLVGDAYKFWRAFR