Protein Info for QEN71_RS40200 in Paraburkholderia sabiae LMG 24235

Annotation: FAD-binding oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01494: FAD_binding_3
  • amino acids 8 to 38 (31 residues), 22.5 bits, see alignment (E = 1.8e-08)
• PF01266: DAO
  • amino acids 10 to 400 (391 residues), 228.8 bits, E=4.5e-71
• PF00890: FAD_binding_2
  • amino acids 10 to 213 (204 residues), 42.3 bits, E=1.8e-14
• PF13450: NAD_binding_8
  • amino acids 12 to 43 (32 residues), 27.4 bits, see alignment (E = 1e-09)

Best Hits

KEGG orthology group: None (inferred from 70% identity to bug:BC1001_2021)

MetaCyc: 44% identical to tyramine oxidase subunit A (Pseudomonas putida U)
Monoamine oxidase. [EC: 1.4.3.4]; 1.4.3.4 [EC: 1.4.3.4]; 1.4.3.4 [EC: 1.4.3.4]

Predicted SEED Role

"D-amino acid dehydrogenase small subunit (EC 1.4.99.1)" in subsystem Pyruvate Alanine Serine Interconversions or Respiratory dehydrogenases 1 (EC 1.4.99.1)

MetaCyc Pathways

• salidroside biosynthesis (3/4 steps found)
• superpathway of phenylethylamine degradation (8/11 steps found)
• phenylethylamine degradation I (1/2 steps found)
• L-tryptophan degradation X (mammalian, via tryptamine) (2/4 steps found)
• L-tryptophan degradation VI (via tryptamine) (1/3 steps found)
• L-phenylalanine degradation IV (mammalian, via side chain) (5/9 steps found)
• dopamine degradation (2/5 steps found)
• phenylethanol biosynthesis (2/5 steps found)
• aromatic biogenic amine degradation (bacteria) (4/8 steps found)
• putrescine degradation III (1/4 steps found)
• serotonin degradation (3/7 steps found)
• noradrenaline and adrenaline degradation (4/13 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Arginine and proline metabolism
• Drug metabolism - cytochrome P450
• Glycine, serine and threonine metabolism
• Histidine metabolism
• Nitrogen metabolism
• Phenylalanine metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 1.4.99.1

Use Curated BLAST to search for 1.4.3.4 or 1.4.99.1

Protein Sequence (420 amino acids)

>QEN71_RS40200 FAD-binding oxidoreductase (Paraburkholderia sabiae LMG 24235)
MQQIPKSTSVAIIGGGIIGISAALALARRGVDVVVFEKGVVAGEQSSRNWGWIRSVGRNP
AELPLSAMANDMWQDIQSGVDVGYRRTGLAYLAASESALAAHQAWLDIARESGANARILS
KGESARLIPSSRRTWAGALYSSTDGVAEPTLATQGIAALAVSLGAKILEGCAARGLDVSA
GRVSGVVTEHGLVKCSSVVLAGGAWSRLLCGNHGIEFPQLKVHASGLQTTPVNAGLDLAI
NGGDFTVRKRSDGGYTVSKLGASVAELTPDSIRLSTKFMGAWVKERKYLRLRIGRRFFDE
LKTPRRFGLDRPTPFEACRTLDPEPNVRMLDEALKQLKSAFAGFSDALPVRAWAGMIDVT
PDALPVISEVSEIPGFFLGSGFSGHGFGIGPAAGSVLANLVTGDRPVVEVRDFSLTRFSS