Protein Info for QEN71_RS37810 in Paraburkholderia sabiae LMG 24235

Annotation: nitrite reductase large subunit NirB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 854 PF07992: Pyr_redox_2" amino acids 1 to 282 (282 residues), 181.9 bits, see alignment E=6.6e-57 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 4 to 796 (793 residues), 1145.7 bits, see alignment E=0 PF00070: Pyr_redox" amino acids 145 to 222 (78 residues), 58.1 bits, see alignment E=3.6e-19 PF18267: Rubredoxin_C" amino acids 316 to 385 (70 residues), 39.9 bits, see alignment E=1.2e-13 PF04324: Fer2_BFD" amino acids 420 to 467 (48 residues), 42.1 bits, see alignment 3.1e-14 PF03460: NIR_SIR_ferr" amino acids 555 to 618 (64 residues), 47.3 bits, see alignment 4.9e-16 PF01077: NIR_SIR" amino acids 628 to 747 (120 residues), 78.7 bits, see alignment E=1.2e-25

Best Hits

Swiss-Prot: 62% identical to NIRB_ECOLI: Nitrite reductase (NADH) large subunit (nirB) from Escherichia coli (strain K12)

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 96% identity to bph:Bphy_5659)

MetaCyc: 62% identical to nitrite reductase (NADH) large subunit (Escherichia coli K-12 substr. MG1655)
RXN-13854 [EC: 1.7.1.15]

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.15 or 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (854 amino acids)

>QEN71_RS37810 nitrite reductase large subunit NirB (Paraburkholderia sabiae LMG 24235)
MKVIVIGHGMVGHKLLECLAEQTAARLDITVLCEEPRPAYDRVHLSEFFSGKSADDLSLV
APGFFDRDDMVLRLNAKAVSIDRDAHTVTTSTGETLAYDKLVIASGSYAFVPPVPGKDRK
DCFVYRTIEDLEAMQECGARSKTGVVIGGGLLGLECAKALRDMGLETHVVEFAPRLMAVQ
VDDGGGKVLRGKIEELGVTVHTQKNTLEIVDGEEGTHRMQFADGSHLDTDMIVFSAGIRP
RDEIARACGLEVGARGGIVIDNQCRTSDADIYAIGECALWNGQIFGLVAPGYEMARVTAK
QLLGEKEAAFAGADMSTKLKLMGVDVASLGDAHASTAGSRTYQFSDERKQVYKKLVVSDC
GKFLLGGVMVGDASEYGTLLQMMLNRIQLPESPEFLILPQADGNAKPALGVDALPESAQI
CSCNNISKGAICASVCAGATTIGDVKNATKAGTACGGCVPLVTQVMKAEMKKQGLAVNNH
LCEHFPFSRQELYHLVRVERIKTFGALLDKHGHGLGCDICKPAVAGILASCWNEFVLKKE
HASLQDSNDYYLANIQRDGTYSVVPRMPGGEVTPEGLIAVGQVAKKYGLYTKITGGQRVD
LFGARVEQLPSIWEELIAAGFESGHAYGKSVRTVKSCVGSTWCRYGVDDSVGLAIDIENR
YKGLRSPHKLKFAVSGCTRECAEAQSKDVGIIATEKGWNLYVCGNGGMKPRHAELIASDL
DRDTLIRYIDRFLMFYVRTADRLQRTSVWRENLEGGLDYLKDVIIHDKLGLAAELEAEMQ
HVVETYECEWKKAVTDPETRKRFRHFVNSESADDNISFVATRGQIRPATPDERQQRPVRI
PVVVEQTAESAETV