Protein Info for QEN71_RS36875 in Paraburkholderia sabiae LMG 24235

Annotation: nitrite/sulfite reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03460: NIR_SIR_ferr
  • amino acids 53 to 83 (31 residues), 25.7 bits, see alignment (E = 8e-10)
  • amino acids 378 to 428 (51 residues), 45.9 bits, 3.7e-16
• PF01077: NIR_SIR
  • amino acids 142 to 295 (154 residues), 147.8 bits, E=1.9e-47
  • amino acids 441 to 585 (145 residues), 61.6 bits, E=6.1e-21

Best Hits

KEGG orthology group: K00381, sulfite reductase (NADPH) hemoprotein beta-component [EC: 1.8.1.2] (inferred from 92% identity to bph:Bphy_5829)

Predicted SEED Role

"Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2)" in subsystem Cysteine Biosynthesis (EC 1.8.1.2)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (12/12 steps found)
• superpathway of sulfate assimilation and cysteine biosynthesis (9/9 steps found)
• assimilatory sulfate reduction I (4/4 steps found)
• assimilatory sulfate reduction III (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (7/10 steps found)

KEGG Metabolic Maps

• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.2

Use Curated BLAST to search for 1.8.1.2

Protein Sequence (596 amino acids)

>QEN71_RS36875 nitrite/sulfite reductase (Paraburkholderia sabiae LMG 24235)
MYQYNEFDKAFLLNRAAQFRDQIERWQDGRLSEDAFRPLRLQNGWYVQRHAPMLRVAVPY
GELSSAQLRVLARVAREYDAPEADVYRQALDAQRKLGTVRLPTHHAHFTTRTNVQFNWIP
LAKAADVMDLLATVNMHGIQTSGNCIRNISCDERAGVAPDEIADPRPFAEIMRQWTTLHP
EFAFLPRKFKIAITGADDDRAATDWHDVGLRLRYGENGELGFRVMVGGGMGRTPVIGTVL
REFLPWQHIMNYIEAVVRVYNQYGRRDNKYKARIKILVKAEGQKYIDEVEEEFRQIVEHD
GGPHTIPHAELERVSAYFVQPATESVSKPSDPTARAKIDDASLRNPPFARWLERNVAQHK
NAQRRIVTLSFKRLLQAPGDASADQLDQVADLVDRFSAGEARVTHTQNIVLPWVHENDLL
ALWEVAREVGLASANVSLLTDMIACPGGDFCALANARSLPIAEAITERYQDMDELEDIGE
VDLHISGCINSCGHHHSGHIGILGVDKDGTEWYQVTLGGSDGSHASGVAQPGKVIGPSFS
AHEVPDVIDAVLRTYVEHRTQSGPRHERFIETVRRIGADPFKAAANAARTPLEVSA