Protein Info for QEN71_RS36450 in Paraburkholderia sabiae LMG 24235
Annotation: L-glutamate gamma-semialdehyde dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 49% identical to PUT2_SCHPO: Probable delta-1-pyrroline-5-carboxylate dehydrogenase (SPBC24C6.04) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)
KEGG orthology group: K00294, 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12] (inferred from 84% identity to bgf:BC1003_6034)MetaCyc: 50% identical to Delta1-pyrroline-5-carboxylate dehydrogenase, mitochondrial subunit (Homo sapiens)
RXN-14116 [EC: 1.2.1.88]; 1.2.1.- [EC: 1.2.1.88]
Predicted SEED Role
"Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization (EC 1.5.1.12)
MetaCyc Pathways
- trans-4-hydroxy-L-proline degradation I (4/5 steps found)
- L-arginine degradation I (arginase pathway) (2/3 steps found)
- L-proline degradation I (2/3 steps found)
- ethene biosynthesis II (microbes) (1/4 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.2.1.88, 1.5.1.12
Use Curated BLAST to search for 1.2.1.88 or 1.5.1.12
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (536 amino acids)
>QEN71_RS36450 L-glutamate gamma-semialdehyde dehydrogenase (Paraburkholderia sabiae LMG 24235) MISGPQFPLPANETEVSFRPGTAAAAELAKALEQRDVVDIPTVIGGKRYFSNDTVEVRAP HDTQRVLARIHRPTESQIAEAIKSSASVAKDWASLTHASRATILHRAADIVATRSRMRIN AATMLGQSKTIDQAEPDSACELIDFLRFNAYNAQRVYAEQPMSVPTAVNRADWRPLEGFV YAVSPFNFTAIGGNLTTAPAIMGNTVLWKPSEKSALANWIFYEALEEAGLPPGVINFVPG DAELTTRVALSSPDLAGIHFTGSSAVFQSLWKGVASKVDSFRTIPRLVGETGGKDFVLAH PSANASEVAIALIRGAFEYQGQKCSAASRAYIPRNLWAAVSQEMRDRLAQLKVGDVAQSD TFMGAVISQASHQKLTRVLAAAREDSGVKVVSGGKTWSDPGYFVEPTVLEVSDPHHALLK EELFGPVLSVFVYDDANWEETLQLIDSTSPYALTGSIFCTDRFALHRAEAALINAAGNLY LNDKPTGAMIGQQPFGGGRASGTNDKAGSYLNLLRWASPRVVKETYLPPRDWQFGA