Protein Info for QEN71_RS33930 in Paraburkholderia sabiae LMG 24235

Annotation: FAD-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01946: Thi4
  • amino acids 12 to 52 (41 residues), 27.2 bits, 4.4e-10
• PF00890: FAD_binding_2
  • amino acids 13 to 52 (40 residues), 20.6 bits, 4.6e-08
• PF01266: DAO
  • amino acids 13 to 359 (347 residues), 224.5 bits, E=5.8e-70

Best Hits

Swiss-Prot: 49% identical to SOXB_CORS1: Sarcosine oxidase subunit beta (soxB) from Corynebacterium sp. (strain P-1)

KEGG orthology group: K00303, sarcosine oxidase, subunit beta [EC: 1.5.3.1] (inferred from 98% identity to bph:Bphy_5914)

MetaCyc: 83% identical to N-methyl glutamate dehydrogenase alpha subunit (Methyloversatilis universalis)
Methylglutamate dehydrogenase. [EC: 1.5.99.5]

Predicted SEED Role

"Sarcosine oxidase beta subunit (EC 1.5.3.1)" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis (EC 1.5.3.1)

MetaCyc Pathways

• methylamine degradation II (3/3 steps found)
• glycine betaine degradation I (6/8 steps found)
• creatinine degradation I (3/4 steps found)
• superpathway of trimethylamine degradation (4/6 steps found)
• creatinine degradation II (2/5 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.5.3.1

Use Curated BLAST to search for 1.5.3.1 or 1.5.99.5

Protein Sequence (395 amino acids)

>QEN71_RS33930 FAD-dependent oxidoreductase (Paraburkholderia sabiae LMG 24235)
MFTRHDTLRRSYDVVIIGAGGHGLASAYYLAKEHGITNVAVLEKGYIGGGNTGRNTTIIR
SNYLTPEGVQFYDESVKLWQDLSQDFDLNLFYSTRGHYTLAHTDSAMRTMRWRAEVNKHY
GVDSEVVGPNEVKKAAPMIDISCGGVAPIMGALYHAPGAVARHDAVAWGYGRGADQRGVE
IHQQTEVLGIDVVGGKVKGVKTSRGYISTNKVLCAVAGSTPRLTDMVGVRTPIFIHPLQA
MVSEPLKPWLDPILVSGSLHVYISQSARGELVMGASLDPYELHSTRSTLDFVEGLTAHML
EMFPFLSQAKVMRQWAGMADMTPDFAPIMGKTPVEGFYLDAGWGTWGFKATPVCGKTMSH
TVANDATHPLIEGFSLDRFRQFSLTGEKGAASVGH