Protein Info for QEN71_RS33820 in Paraburkholderia sabiae LMG 24235
Annotation: aspartate ammonia-lyase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 60% identical to ASPA_PSEAE: Aspartate ammonia-lyase (aspA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K01744, aspartate ammonia-lyase [EC: 4.3.1.1] (inferred from 94% identity to bph:Bphy_5639)MetaCyc: 57% identical to aspartate ammonia-lyase (Escherichia coli K-12 substr. MG1655)
Aspartate ammonia-lyase. [EC: 4.3.1.1]
Predicted SEED Role
"Aspartate ammonia-lyase (EC 4.3.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 4.3.1.1)
MetaCyc Pathways
- L-glutamate degradation II (2/2 steps found)
- superpathway of L-aspartate and L-asparagine biosynthesis (3/4 steps found)
- L-aspartate degradation II (aerobic) (2/3 steps found)
- L-aspartate degradation III (anaerobic) (2/3 steps found)
- L-canavanine degradation II (2/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.3.1.1
Use Curated BLAST to search for 4.3.1.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (482 amino acids)
>QEN71_RS33820 aspartate ammonia-lyase (Paraburkholderia sabiae LMG 24235) MFNTTERTERIEHDLLGDLAVPDAAYYGVHTLRALANFPITGIPISTYPNLVNALASVKE AAAMANHDLGLLAGHKMNAIVHACREIRAGVAHDQFVVDVIQGGAGTSTNMNANEVIANL ALEHLGHRRGDYSMLHPNEDVNLGQSTNDVYPTALKIATYMGIVQLVHAMGVLRHAFERK AEEFRDDLKMGRTQLQDAVPMTLGQEFSTFAVMLGEDEERLLEASKLICEINLGATAIGT GINTSPDYAPLACRHLASITGIPVTTSPNLVEATQDVGSFVQLSGVLKRVAIKLSKTCND LRLLSSGPRAGFGEINLPPVQAGSSIMPGKVNPVIPEVVNQIAFEVIGNDVTVSFAAEAG QLQLNAFEPLIAHSIFKSVAHLRAGCLTLASRCVDGITANRDRLHSLVENSIGIVTALNP YIGYTNATQVAQQALLSGKSVAEIVIETGLLSRAQLDEILQPAMLTEPRRALAFDSGALE QG