Protein Info for QEN71_RS30040 in Paraburkholderia sabiae LMG 24235

Annotation: EAL domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 978 transmembrane" amino acids 27 to 49 (23 residues), see Phobius details amino acids 316 to 336 (21 residues), see Phobius details PF02743: dCache_1" amino acids 55 to 299 (245 residues), 46.2 bits, see alignment E=1.5e-15 PF00672: HAMP" amino acids 334 to 394 (61 residues), 25.5 bits, see alignment 4.9e-09 TIGR00229: PAS domain S-box protein" amino acids 425 to 541 (117 residues), 40.2 bits, see alignment E=3.4e-14 PF00989: PAS" amino acids 425 to 532 (108 residues), 37 bits, see alignment E=1.1e-12 PF08448: PAS_4" amino acids 429 to 536 (108 residues), 47.6 bits, see alignment E=6.5e-16 PF13426: PAS_9" amino acids 439 to 535 (97 residues), 31.8 bits, see alignment E=4.9e-11 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 545 to 707 (163 residues), 154.9 bits, see alignment E=1.6e-49 PF00990: GGDEF" amino acids 547 to 704 (158 residues), 184.7 bits, see alignment E=3.8e-58 PF00563: EAL" amino acids 725 to 960 (236 residues), 248.2 bits, see alignment E=2.7e-77

Best Hits

KEGG orthology group: None (inferred from 93% identity to bph:Bphy_7217)

Predicted SEED Role

"diguanylate cyclase/phosphodiesterase with PAS/PAC sensor(s)"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (978 amino acids)

>QEN71_RS30040 EAL domain-containing protein (Paraburkholderia sabiae LMG 24235)
MSSIIASLQRAFEYLSQDLLRRVEIRYRLITAFILLSLLPVLISGYISYAESTAAIKEKA
EIFSKEVVKQVSRNVVLRMEQIETESSLLVLSDQVQAALMKVANGNAKEQSEARQDMTRL
LLDHYGSVDFINEKYLLDDHDRIMDTQAFSQLTRGVMRFVRQAPDHNGRPYWGSYDNGVG
QQNLGMVRAVIGKSNNRRLGNLVLVIRPEHFSTIFNDVASGTGTQICVVDMRTNKLIVRA
DDTSASADTTVAPSLIDRITHNNEALGEPNGFVSYEGKDRAGYFAAYTRIPGTTWFVVST
IPEKKLTAEAQSVRNQIVLVGISGFLLSIFFAYFISHSISAPLKNLVRKMHDTGSDAGAE
ADALAEEATVDGNGQDELGRLEARFERMRAAIRQKIHKINEINASLEQTVAERTAELVSR
ELESRTLIENSPDTITRYDRDLRRTYANPAFCASAGRSLSEALGKRPSEIPGGTNALIYE
RKINEAISSGKSGQFELRWLSKDGQEQCSHIRLTPEIDPSGNVNSVLAVGRDLSDRMAFE
AAIWQQANFDTLTQLPNRQMFQNRLEQEAKIAQRSGHRMALMLIDLDRFKEVNDSLGHDT
GDTLLIEAARRITSCVRETDTVARLGGDEFTVILPDIDNAGSIERIARSIIATLCEPFAL
GPDEAFISASIGVTVYPDDARELDVLFKNADQAMYAAKNAGRNRLNYFTPDLQVAAEKRL
RLTSDLRTALPHEQFRLYYQPIVDLATGDIFKAEALIRWLHPERGMVSPLDFIPLAEDTG
LIVPIGDWVFRQAVQQAKLWRTRFHPSFQISVNMSPVQIRQDNQLGTQWSDYLTREGMPG
QSVAIEITEGLLLHADPDIDERLATFRNAGIRISIDDFGTGYSSLAYLKRFDIDFLKIDR
SFVQNLGFDADNQALCEAMVVLAHKLGLKVIAEGVETVEQRDFLTAVGCDFAQGYLYSQP
VPADQFEALVWPRIEASI