Protein Info for QEN71_RS29945 in Paraburkholderia sabiae LMG 24235

Annotation: 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00378: ECH_1
  • amino acids 16 to 198 (183 residues), 118 bits, E=1.2e-37
• PF16113: ECH_2
  • amino acids 28 to 192 (165 residues), 79.9 bits, E=6.5e-26
• PF03446: NAD_binding_2
  • amino acids 303 to 417 (115 residues), 21.8 bits, E=4.1e-08
• PF02737: 3HCDH_N
  • amino acids 303 to 478 (176 residues), 200.3 bits, E=6.6e-63
• PF00725: 3HCDH
  • amino acids 483 to 576 (94 residues), 80.1 bits, E=3.5e-26
  • amino acids 612 to 696 (85 residues), 26 bits, E=2.8e-09

Best Hits

KEGG orthology group: K07516, 3-hydroxyacyl-CoA dehydrogenase [EC: 1.1.1.35] (inferred from 91% identity to bph:Bphy_7224)

Predicted SEED Role

"Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation or Butanol Biosynthesis (EC 1.1.1.35, EC 4.2.1.17, EC 5.1.2.3, EC 5.3.3.8)

MetaCyc Pathways

• oleate β-oxidation (28/35 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
• adipate degradation (5/5 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• fatty acid salvage (5/6 steps found)
• pyruvate fermentation to butanol II (engineered) (5/6 steps found)
• phenylacetate degradation I (aerobic) (7/9 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (4/5 steps found)
• adipate biosynthesis (4/5 steps found)
• fatty acid β-oxidation IV (unsaturated, even number) (4/5 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• fatty acid β-oxidation III (unsaturated, odd number) (1/1 steps found)
• superpathway of phenylethylamine degradation (8/11 steps found)
• benzoyl-CoA degradation I (aerobic) (5/7 steps found)
• fatty acid β-oxidation I (generic) (5/7 steps found)
• L-isoleucine degradation I (4/6 steps found)
• propanoate fermentation to 2-methylbutanoate (4/6 steps found)
• oleate β-oxidation (thioesterase-dependent, yeast) (1/2 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• glycerol degradation to butanol (11/16 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (3/5 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• L-valine degradation I (5/8 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (4/7 steps found)
• pyruvate fermentation to butanoate (4/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (9/14 steps found)
• oleate β-oxidation (reductase-dependent, yeast) (1/3 steps found)
• 3-phenylpropanoate degradation (6/10 steps found)
• 6-gingerol analog biosynthesis (engineered) (3/6 steps found)
• methyl ketone biosynthesis (engineered) (3/6 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (5/9 steps found)
• valproate β-oxidation (5/9 steps found)
• fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) (2/5 steps found)
• pyruvate fermentation to butanol I (4/8 steps found)
• oleate β-oxidation (isomerase-dependent, yeast) (1/4 steps found)
• (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (10/17 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (7/13 steps found)
• benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
• gallate degradation III (anaerobic) (5/11 steps found)
• 9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (4/10 steps found)
• 2-methylpropene degradation (2/8 steps found)
• methyl tert-butyl ether degradation (3/10 steps found)
• L-glutamate degradation VII (to butanoate) (4/12 steps found)
• L-tryptophan degradation III (eukaryotic) (6/15 steps found)
• 10-cis-heptadecenoyl-CoA degradation (yeast) (2/12 steps found)
• 10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (2/12 steps found)
• (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
• docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (4/17 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (3/16 steps found)
• cholesterol degradation to androstenedione I (cholesterol oxidase) (3/17 steps found)
• toluene degradation VI (anaerobic) (3/18 steps found)
• Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
• androstenedione degradation I (aerobic) (6/25 steps found)
• platensimycin biosynthesis (6/26 steps found)
• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (3/22 steps found)
• superpathway of testosterone and androsterone degradation (6/28 steps found)
• androstenedione degradation II (anaerobic) (4/27 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (9/42 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (9/47 steps found)
• superpathway of cholesterol degradation III (oxidase) (5/49 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Bile acid biosynthesis
• Biosynthesis of plant hormones
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation
• alpha-Linolenic acid metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.35, 4.2.1.17, 5.1.2.3, 5.3.3.8

Use Curated BLAST to search for 1.1.1.35 or 4.2.1.17 or 5.1.2.3 or 5.3.3.8

Protein Sequence (706 amino acids)

>QEN71_RS29945 3-hydroxyacyl-CoA dehydrogenase NAD-binding domain-containing protein (Paraburkholderia sabiae LMG 24235)
MTPTPTTAPVSHELRGNILLVTVDNPPVNALGVDVPRGLVAAIEAAEANAAARAVLILGA
GRNFIAGADIREFGKPPQMPTLPDVCNRIEACSKPVVVAIHGAALGGGLEIALAAHYRLA
VAGAKLGLPEVLLGLLPGAGGTQRASRLIGAEAALSLMLSGKHIGEQQALKLGLVDRVGQ
SDDVLAEGLAYTQQLLVDHAPVRRTRDAQALADKDASAAAIAAARADAAKKSRGLLSPLK
IVDCVEAALTLPFEDGLRFERAQFLECLDSPQRSALVHAFQAEREVQKAPETRSAQPRPV
ASVGVVGGGTMGAGIAVALLDAGLPVTMVERDDASLARGRAHVEKVYDGLIAKGRLTDDA
KAKKLARFSGSTSYEALAEVDVVIEAVFEDMAVKQAVFAELDRVCKQGAVLATNTSYLDI
DAIAASISRPQDVIGLHFFSPAHIMKLLEVVVPARVSADVVATAFDLAKKLRKVPVRAGV
CDGFIGNRMLAVYRAAADHLMEDGASPYQIDKAVRELGFPMGPYQVIDLAGGDIGWATRK
RRAATRNPDARYVQIPDRLCERGWFGQKTARGFYRYEEGKRGGMPDPEVEAIIDTERERA
GVTPRAFTDEQIVRRYMAAMINEGANVIHERIALRPLDVDVTLMNGFGFPAYRGGPMHYA
DTIGLPALLADIREFEKEDPIFWKPSPLLVELVERGATFASLNQAA