Protein Info for QEN71_RS29465 in Paraburkholderia sabiae LMG 24235

Annotation: glutamine amidotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00117: GATase
  • amino acids 23 to 188 (166 residues), 64.5 bits, E=1e-21

Best Hits

KEGG orthology group: K01951, GMP synthase (glutamine-hydrolysing) [EC: 6.3.5.2] (inferred from 96% identity to bph:Bphy_3062)

Predicted SEED Role

"GMP synthase - Glutamine amidotransferase domain"

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• L-glutamate and L-glutamine biosynthesis (7/7 steps found)
• superpathway of purine nucleotide salvage (12/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Drug metabolism - other enzymes
• Glutamate metabolism
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.2

Use Curated BLAST to search for 6.3.5.2

Protein Sequence (237 amino acids)

>QEN71_RS29465 glutamine amidotransferase (Paraburkholderia sabiae LMG 24235)
MLHEVLAIRHVHFEDLGSLERVLGERGRPVRYLDVGFARIEAPDPVMPSLMVILGGPISA
YDDDRYPTIAPLVAMIEKRIAAGLPTLGICLGAQLIARVLGAKVYPSGQTEIGWTPLTLT
DAGKQSPLRHLDAAHTSMLHWHGDTFDLPQGAVHLASTPACENQAFAWGKHVLALQCHPE
IRTDRFEPWLIGNAGEIAGHGIDVRTLRADTAKHGPKLEAAATSMFGEWLDQVAAQP