Protein Info for QEN71_RS29260 in Paraburkholderia sabiae LMG 24235

Annotation: primosomal protein N'

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 752 PF17764: PriA_3primeBD" amino acids 12 to 107 (96 residues), 88.8 bits, see alignment E=5.1e-29 PF04851: ResIII" amino acids 210 to 377 (168 residues), 51.8 bits, see alignment E=2.9e-17 PF00270: DEAD" amino acids 213 to 377 (165 residues), 60.3 bits, see alignment E=6.3e-20 TIGR00595: primosomal protein N'" amino acids 232 to 750 (519 residues), 550.2 bits, see alignment E=2.1e-169 PF18319: PriA_CRR" amino acids 468 to 493 (26 residues), 37.1 bits, see alignment (E = 7.2e-13) PF00271: Helicase_C" amino acids 525 to 612 (88 residues), 29.8 bits, see alignment E=1.8e-10 PF18074: PriA_C" amino acids 655 to 749 (95 residues), 54.4 bits, see alignment E=5.4e-18

Best Hits

KEGG orthology group: K04066, primosomal protein N' (replication factor Y) (superfamily II helicase) [EC: 3.6.4.-] (inferred from 95% identity to bph:Bphy_3022)

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (752 amino acids)

>QEN71_RS29260 primosomal protein N' (Paraburkholderia sabiae LMG 24235)
MQVEALTDTYVRVALDHPLPTLFDYRCDAALAPTPGMLVSVPFGKRHIVGLVMEVTAHSD
VPADRLKSVHSICTACPPLSKHWLQLAQFAADYYQRGIGEVALPALPQALRDAARWTRLF
APEERYRLLPAGRAALPDALPARATALRRLAAALAETESLPAVDARALHAKATATLEAWQ
AEGWVALDVIDPAAPRLSLDHSPQNASLPTLTDEQTTAVEAIREAQGFAPFLLHGVTGSG
KTEVYLRALAGLLAANPQAQALVLVPEINLTPQFEAAFRARFASMDDSEIVTLHSGLAEG
ERARNWFAAHTGRARIVLGTRLAVMASLPHLAIIVVDEEHDPAYKQQEGLRYSARDLAIW
RAKQLAVPVVLGSATPSLESWWQVDQGRYKRLTLSRRAVADAVLPTVRLVDLEDERRRGR
ASVEGLSGPLIAALKARLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYVVLHKP
ERALRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRIEEALAGAVPGARVLRIDADSTRR
KGSAQALFSDVHAGEVDILVGTQMIAKGHDFRRVSLVGVLNADTALFSHDFRASERLFAQ
LMQVSGRAGRAGLPGEVLVQTRYPTHALYHALARQDYVGFANSTLSERRDAHLPPFVYQG
MLRAEGRTLDAALTFLQQAAAALSEIPAASRVTVYDAVPLTIVKVMHVHRAQLLVESASR
AALQATLRAWQPLLRTMKGVLRWSIEVDPLDI