Protein Info for QEN71_RS29215 in Paraburkholderia sabiae LMG 24235

Annotation: aminomethyl-transferring glycine dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 976 transmembrane" amino acids 344 to 364 (21 residues), see Phobius details PF02347: GDC-P" amino acids 28 to 454 (427 residues), 598.1 bits, see alignment E=2e-183 amino acids 493 to 766 (274 residues), 43.7 bits, see alignment E=3.7e-15 TIGR00461: glycine dehydrogenase" amino acids 29 to 969 (941 residues), 1471.6 bits, see alignment E=0 PF01212: Beta_elim_lyase" amino acids 564 to 703 (140 residues), 27.8 bits, see alignment E=3.2e-10 PF00266: Aminotran_5" amino acids 598 to 724 (127 residues), 23 bits, see alignment E=7.5e-09 PF21478: GcvP2_C" amino acids 797 to 918 (122 residues), 202.4 bits, see alignment E=3.3e-64

Best Hits

Swiss-Prot: 59% identical to GCSP2_COLP3: Glycine dehydrogenase (decarboxylating) 2 (gcvP2) from Colwellia psychrerythraea (strain 34H / ATCC BAA-681)

KEGG orthology group: K00281, glycine dehydrogenase [EC: 1.4.4.2] (inferred from 63% identity to ajs:Ajs_1936)

Predicted SEED Role

"Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2)" in subsystem Glycine and Serine Utilization or Glycine cleavage system or Photorespiration (oxidative C2 cycle) (EC 1.4.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (976 amino acids)

>QEN71_RS29215 aminomethyl-transferring glycine dehydrogenase (Paraburkholderia sabiae LMG 24235)
MKLEHPDRLMNRTPLSLAALEVHDAFAERHIGPDGADQQSMLEALGFASRAALMDAVIPK
SIRRQDALPLGPFGQPKSEAEALAALRELADKNQVFRSYIGQGYYNAHTPTVILRNVLEN
PAWYTAYTPYQPEISQGRLEALLNYQQMIVDLTGLAISNASLLDEATAAAEAMTLLQRVG
KPKSNIFYVADDVLPQTIEVVKTRATPVGIEVKVGPAAEAATSNAFGVLLQYPGVNGDVR
DYRALADAIHAAGGHVVAAADLLALTVLTPPGEWGADVAVGNSQRFGVPVGFGGPHAAYL
AVRDEFKRQMPGRLVGVTVDAQGNPALRLALQTREQHIRREKATSNVCTAQALLAIMASM
YAVYHGPRGLKMIAQRVNRIAALLAEGAKQLGYTLVNEHFFDTLTFETGPRTQALHDAAT
ARRINLRRVSDTRVGISIDETTTRADLADLLAAFAQAAFVEAVPQIDELDAKLGQSNTFP
ATLERESAHLTHHVFNRHHSETEMLRYLRSLSDKDLALDRSMIPLGSCTMKLNATSEMLP
VTWPEFGQIHPFAPAEQTVGYREMIDQLEQMLVAATGYAAVSLQPNAGSQGEYAGLLIIH
AYHESRGEGHRNVCLIPASAHGTNPASAQMAGMQVVVVACDAQGNIDVEDLKKKAEQHSD
KLAAIMITYPSTHGVFEQNVREVCEIVHAHGGQVYVDGANMNAMVGLAAPGQFGGDVSHL
NLHKTFCIPHGGGGPGVGPVAVGAHLAKFLPNQTSTGYERDANGIGAVSAAPYGSASILP
ISWMYIAMMGAKNLTAATETAILNANYVANKLAPHYPVLYSGPGGLVAHECILDLRPIKE
TSGISVDDVAKRLMDYGFHAPTMSFPVPGTLMVEPTESESKEELDRFIEAMIAIRNEIRE
VEEGRSDREDNPLKHAPHTAAVVVANDWKHGYARETAAYPLPTLVAKKYWPPVGRADNAY
GDRNLFCSCVPVADYE