Protein Info for QEN71_RS28590 in Paraburkholderia sabiae LMG 24235

Annotation: bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 1 to 451 (451 residues), 623.3 bits, E=1.2e-191
• PF01128: IspD
  • amino acids 2 to 131 (130 residues), 36.4 bits, E=1.3e-12
• PF12804: NTP_transf_3
  • amino acids 4 to 130 (127 residues), 82.6 bits, E=8.9e-27
• PF00483: NTP_transferase
  • amino acids 4 to 221 (218 residues), 54.7 bits, E=2.8e-18
• PF00132: Hexapep
  • amino acids 391 to 423 (33 residues), 29.9 bits, see alignment (E = 7.9e-11)
• PF14602: Hexapep_2
  • amino acids 391 to 423 (33 residues), 25.2 bits, see alignment (E = 2.7e-09)

Best Hits

Swiss-Prot: 96% identical to GLMU_PARP8: Bifunctional protein GlmU (glmU) from Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 96% identity to bph:Bphy_2891)

MetaCyc: 55% identical to fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.23]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.23, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• peptidoglycan recycling I (10/14 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (6/9 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (8/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Protein Sequence (453 amino acids)

>QEN71_RS28590 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU (Paraburkholderia sabiae LMG 24235)
MNIVILAAGTGKRMRSALPKVLHPLAGRPLLAHVIDTARTLNPSRLVVVVGHGAEQVRTA
VAAPDVQFAVQEQQLGTGHAVQQALPLLDPSAPTLVLYGDVPLTKASTLKRLTDAAGHDG
YGVLTVTLDDPTGYGRIVRDAQDKVERIVEQKDATPDQLKIAEINTGIVVMPTKRLDAWL
SSLKNDNAQGEFYLTDVVELAIEAGIEVVTAQPDEEWETLGVNSKQQLAELERIHQRNVA
DELLVAGVTIADPARIDVRGTLECGRDVSIDVNCVFEGKVTLADNVSIGPNCVIRNASIG
AGTRIDAYTHIEGAQIGAQAVLGPYARLRPGATLADETHIGNFVEVKNAVLGHGSKANHL
SYIGDSDVGARVNIGAGTITCNYDGANKFRTVIEDDVFVGSDTQLVAPVRVGRGVTIAAG
TTVWKDVEEGLLVLNEKSQIGKTGYVRPTKKKS