Protein Info for QEN71_RS28585 in Paraburkholderia sabiae LMG 24235

Annotation: glutamine--fructose-6-phosphate transaminase (isomerizing)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01135: glutamine-fructose-6-phosphate transaminase (isomerizing)
  • amino acids 2 to 605 (604 residues), 815.8 bits, E=1.1e-249
• PF13230: GATase_4
  • amino acids 54 to 135 (82 residues), 23.7 bits, E=4.1e-09
• PF13522: GATase_6
  • amino acids 65 to 188 (124 residues), 73.9 bits, E=2.5e-24
• PF13537: GATase_7
  • amino acids 81 to 191 (111 residues), 56.4 bits, E=5.8e-19
• PF01380: SIS
  • amino acids 286 to 412 (127 residues), 90.3 bits, E=1.9e-29
  • amino acids 458 to 585 (128 residues), 87 bits, E=1.9e-28

Best Hits

Swiss-Prot: 74% identical to GLMS_RALSO: Glutamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K00820, glucosamine--fructose-6-phosphate aminotransferase (isomerizing) [EC: 2.6.1.16] (inferred from 97% identity to bph:Bphy_2890)

Predicted SEED Role

"Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)" in subsystem Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.6.1.16)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (6/9 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis III (2/6 steps found)
• CMP-legionaminate biosynthesis I (2/10 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (8/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Glutamate metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.16

Use Curated BLAST to search for 2.6.1.16

Protein Sequence (605 amino acids)

>QEN71_RS28585 glutamine--fructose-6-phosphate transaminase (isomerizing) (Paraburkholderia sabiae LMG 24235)
MCGIVGAVAQRNIVSVLIEGLRRLEYRGYDSCGVAVLANGEPKRARSVARVADLDDQVRE
TRLEGITGIAHTRWATHGAPVTDNAHPIFSRNELALVHNGIIENYETLREMLRGKGYEFV
SQTDTEVIAHLIHSMYRGDLFAAVREATAQLHGAYAIAVLHKNQPNTVVGARQGSPLVVG
LGEGENFLASDALALAGSTDRFIFLEEGDVCELTLEKVRVFDRDGKPADREVRQVAAYGG
AVELGPYRHFMQKEIFEQPRAITDTIPQADAFDASLFGENAQQAFSEIDNLLILACGTSY
YSGLTAKYWLESVAKIPTQVEIASEYRYRDSVPNPKSLVVVISQSGETADTLAALKHAQS
LGHKHTLSVCNVGTSAMVRLTELSFLTHAGREIGVASTKAFTTQLVALFVLAATLGKMRG
HVSATQEADYIRQLRHLPAALNSVLALEPQIIAWSEEFSRKENALFLGRGLHYPIALEGA
LKLKEISYIHAEAYPAGELKHGPLALVTEAMPVVTVAPNDALLEKLKSNIQEVRARGGQL
YVFADSDTKIVNDEGLHVIRMPEHYGLLSPILHVVPLQMLAYHTACARGTDVDKPRNLAK
SVTVE