Protein Info for QEN71_RS26640 in Paraburkholderia sabiae LMG 24235

Annotation: tRNA guanosine(34) transglycosylase Tgt

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 402 TIGR00430: tRNA-guanine transglycosylase" amino acids 28 to 394 (367 residues), 525.1 bits, see alignment E=9.3e-162 TIGR00449: tRNA-guanine family transglycosylase" amino acids 28 to 394 (367 residues), 499.4 bits, see alignment E=5.5e-154 PF01702: TGT" amino acids 35 to 396 (362 residues), 535.8 bits, see alignment E=2.6e-165

Best Hits

Swiss-Prot: 82% identical to TGT_CUPMC: Queuine tRNA-ribosyltransferase (tgt) from Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 / CH34)

KEGG orthology group: K00773, queuine tRNA-ribosyltransferase [EC: 2.4.2.29] (inferred from 98% identity to bph:Bphy_2525)

MetaCyc: 64% identical to tRNA-guanine transglycosylase (Escherichia coli K-12 substr. MG1655)
tRNA-guanine transglycosylase. [EC: 2.4.2.29]

Predicted SEED Role

"tRNA-guanine transglycosylase (EC 2.4.2.29)" in subsystem Queuosine-Archaeosine Biosynthesis (EC 2.4.2.29)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.29

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (402 amino acids)

>QEN71_RS26640 tRNA guanosine(34) transglycosylase Tgt (Paraburkholderia sabiae LMG 24235)
MTNGHNNAARADGAYLGDHVRPDNGLKFELLKTDGLARRGRLTLNHGVVETPIFQPVGTY
GTVKAIQPRELEEIRAQIILGNTFHLWLRPGLETIAAHGGLHGFMGWKRPILTDSGGFQV
FSLGDLRKITEDGVTFASPINGDKLFLSPEVSMQIQKVLNSDIVMQFDECTPYATNGVPT
SHKEAADSMRMSMRWAKRSIDEFNKLGNPNALFGIVQGGMFEDLRDESLAGLSEIGFHGL
AIGGLSVGEPKEDMMRVLNHIGPKLPADMPHYLMGVGTPEDLVAGVAAGVDMFDCVMPTR
NARNGWLFTRFGDIKIRNATHKNSMRPLDEQCGCYTCRNFTRGYLHHLHRVGEILGAQLN
TIHNLHYYLELMQEMRDAIDAQMFGAFRKQFHENRARGTDQA