Protein Info for QEN71_RS25870 in Paraburkholderia sabiae LMG 24235

Annotation: D-xylose ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 342 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details TIGR02634: D-xylose ABC transporter, D-xylose-binding protein" amino acids 38 to 339 (302 residues), 492.5 bits, see alignment E=2.9e-152 PF13407: Peripla_BP_4" amino acids 40 to 296 (257 residues), 219.8 bits, see alignment E=4.8e-69 PF00532: Peripla_BP_1" amino acids 59 to 246 (188 residues), 35.2 bits, see alignment E=9.9e-13

Best Hits

Swiss-Prot: 59% identical to XYLF_ECOLI: D-xylose-binding periplasmic protein (xylF) from Escherichia coli (strain K12)

KEGG orthology group: K10543, D-xylose transport system substrate-binding protein (inferred from 99% identity to bph:Bphy_2341)

MetaCyc: 59% identical to xylose ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
ABC-33-RXN [EC: 7.5.2.10, 7.5.2.13]

Predicted SEED Role

"Xylose ABC transporter, periplasmic xylose-binding protein XylF" in subsystem Xylose utilization

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.5.2.10 or 7.5.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (342 amino acids)

>QEN71_RS25870 D-xylose ABC transporter substrate-binding protein (Paraburkholderia sabiae LMG 24235)
MKSAMRRSVLSSLVCAAMAAGLTMVAPLAHASKDKPEIGFCIDDLRVERWSRDRDYFVAA
ATKLGAKVSVQSADASEARQISQIENLISRGVDVIVIVPFNSKTLGNVVAEAHKAGIKVV
SYDRLILDADVDAYISFDNEKVGELQAQGVFNAQPKGNYFLLGGAPTDNNAKMLREGQMK
VLKPAIDRGDVKIVGQQWVPEWSAATALRITEDALTANNNKIDAIVASNDGTAGGAIQAL
AAQKMAGKVPVSGQDADLAAVKRVIAGTQTMTVYKPLKLIAGEAAKLAVDLAKGDKPAFN
AKYDNGKKQVDTVLLQPTLLTKSNVDVVIKDGFYTQSQLAGN