Protein Info for QEN71_RS25205 in Paraburkholderia sabiae LMG 24235
Annotation: leucyl aminopeptidase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 99% identical to AMPA_PARP8: Probable cytosol aminopeptidase (pepA) from Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815)
KEGG orthology group: K01255, leucyl aminopeptidase [EC: 3.4.11.1] (inferred from 99% identity to bph:Bphy_2225)MetaCyc: 51% identical to aminopeptidase A/I (Escherichia coli K-12 substr. MG1655)
Cytosol nonspecific dipeptidase. [EC: 3.4.13.18]; Leucyl aminopeptidase. [EC: 3.4.13.18, 3.4.11.1, 3.4.11.9]
Predicted SEED Role
"Cytosol aminopeptidase PepA (EC 3.4.11.1)" (EC 3.4.11.1)
MetaCyc Pathways
- γ-glutamyl cycle (5/6 steps found)
- glutathione degradation (DUG pathway) (2/2 steps found)
- muropeptide degradation (1/4 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 3.4.11.9
Use Curated BLAST to search for 3.4.11.1 or 3.4.11.9 or 3.4.13.18
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (503 amino acids)
>QEN71_RS25205 leucyl aminopeptidase (Paraburkholderia sabiae LMG 24235) MDFSIKACDWSKGSTNGFLTGKSDCIVIGVFESQTLSGAALEIDAATKGLLTRIVKAGDM DGKIGSTVFLHEVQGIGASRVLLVGLGKQDAFTQKVYGDAVRTAWRAILGTKVAQVTFTL AQAPVKERSSDWAVRAAILALRELTYRFTQMKSKPDTSTRALKRIVFSVDAADEKLAKVA AKQGVALANGMDLTKDLGNLPGNVCTPTYLGNTAKKLAKDWKLKVEVLGQKQIEALKMGS FLAVTQGSVEPPQFIVLQYQGGGAKAAPVVLVGKGITFDTGGISLKPGDSMDEMKYDMCG AGSVLGTLRAVAEMGLKLNVVGIIPTCENMPAGNATKPGDIVTSMKGLTIEVLNTDAEGR LILCDALTYAERFKPAAVIDIATLTGACIIALGHHNSGLFSKDDALAGELLDASKEASDP AWRLPLDDEYQDQLKSNFADIANIGGRPAGSVTAACFLSRFTEAYPWAHLDIAGTAWKSG AAKGATGRPVPLLAQFLIDRAAQ