Protein Info for QEN71_RS24970 in Paraburkholderia sabiae LMG 24235
Annotation: argininosuccinate lyase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 97% identical to ARLY_PARP8: Argininosuccinate lyase (argH) from Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815)
KEGG orthology group: K01755, argininosuccinate lyase [EC: 4.3.2.1] (inferred from 97% identity to bph:Bphy_2183)MetaCyc: 41% identical to argininosuccinate lyase (Homo sapiens)
Argininosuccinate lyase. [EC: 4.3.2.1]
Predicted SEED Role
"Argininosuccinate lyase (EC 4.3.2.1)" in subsystem Arginine Biosynthesis extended (EC 4.3.2.1)
MetaCyc Pathways
- L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
- superpathway of arginine and polyamine biosynthesis (15/17 steps found)
- L-arginine biosynthesis I (via L-ornithine) (9/9 steps found)
- L-arginine biosynthesis III (via N-acetyl-L-citrulline) (8/9 steps found)
- urea cycle (4/5 steps found)
- nitric oxide biosynthesis II (mammals) (2/3 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
- canavanine biosynthesis (2/4 steps found)
- L-arginine biosynthesis IV (archaea) (4/9 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Arginine and proline metabolism
- Urea cycle and metabolism of amino groups
Isozymes
Compare fitness of predicted isozymes for: 4.3.2.1
Use Curated BLAST to search for 4.3.2.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (468 amino acids)
>QEN71_RS24970 argininosuccinate lyase (Paraburkholderia sabiae LMG 24235) MTSQLHKKGEAWSARFSEPMSELVKRYTSSVFFDKRLAFVDIEGSLAHASMLAAQKIIGA DDLAAIQRGMAQIKGEIERGEFEWQLDLEDVHLNIEARLTALIGDAGKRLHTGRSRNDQV ATDIRLWLRGEIDRIGDLLKDLRVALLDLAEKNADTIMPGFTHLQVAQPVTFGHHLLAYV EMFSRDAERMLDTRKRVNRLPLGAAALAGTSYPIDRHAVAKTLSFDGICANSLDAVSDRD FAIEFTAASALVMTHVSRFSEELVLWMSPRVGFIDIADRFCTGSSIMPQKKNPDVPELAR GKTGRVNGHLMALLTLMKGQPLAYNKDNQEDKEPLFDTVDTVADTLRIFAEMVAGITVKP DAMRSAALQGFSTATDLADYLVKRGLPFRDAHEAVAHAVKVCDDRGCDLADLTLDEMKAE LPNVAALLGDDVFGYLTLEGSVASRNHPGGTAPDQVRAAIAAVRATLG