Protein Info for QEN71_RS23930 in Paraburkholderia sabiae LMG 24235

Annotation: error-prone DNA polymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1049 TIGR00594: DNA polymerase III, alpha subunit" amino acids 13 to 994 (982 residues), 543 bits, see alignment E=8.1e-167 PF02811: PHP" amino acids 18 to 113 (96 residues), 54.5 bits, see alignment E=4.5e-18 PF07733: DNA_pol3_alpha" amino acids 294 to 548 (255 residues), 294.4 bits, see alignment E=2.2e-91 PF17657: DNA_pol3_finger" amino acids 551 to 717 (167 residues), 198.3 bits, see alignment E=1.4e-62 PF14579: HHH_6" amino acids 790 to 881 (92 residues), 72.9 bits, see alignment E=5.2e-24 PF01336: tRNA_anti-codon" amino acids 962 to 1030 (69 residues), 28.4 bits, see alignment 3.2e-10

Best Hits

Swiss-Prot: 68% identical to DNAE2_BURPS: Error-prone DNA polymerase (dnaE2) from Burkholderia pseudomallei (strain K96243)

KEGG orthology group: K14162, error-prone DNA polymerase [EC: 2.7.7.7] (inferred from 72% identity to bgl:bglu_4p0900)

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1049 amino acids)

>QEN71_RS23930 error-prone DNA polymerase (Paraburkholderia sabiae LMG 24235)
MDTTFNVLPAYAELFCLSNFTFLHGASHAEELAERASQLGYSGLAITDECSLAGVVRAHV
AAKEKGLPLVIGSYFRLRNADGTPAFGLILLAQNREGYGNLSELITLARTRAPKGEYRLT
PHDLSRPDRENVHLRGMPDCLAILVPDFPANEEVLDAQIEWLDETFPGRAWVGLVLHQRA
MDDIHRGSVEYVANKQNVPVVALGHVVMHVRSRKPLQDTMTAIRVGRPVHECGYELAPNA
EQHLRSRLRIANLYPDHTIRETISVLSRCAFSLDQLRYEYPDELVPEGHTPTAYLRQETY
IGAQQRFPSGIPFNVQEQIEHELALIADLGYEPYFLTVYDIVRYARSQHILCQGRGSAAN
SAVCYCLGVTEVDPARGNMLFERFISKERGEPPDIDVDFEHQRREEVIQYIYRKYGRDRA
AIAAAVSTYRPRGALRETGKALGVDPQIVDTVAKSHHWFDTSHDLLKRFEESGLDPEKPL
IQAWARLASQLLNFPRHLSQHSGGFVISRGKLTRLVPVENAAMADRSVIEWDKDDLEALG
LLKVDVLALGMLSAIRRTLDLVSQQRGEIFEMQHIPPEDKATYDMISNADTIGVFQIESR
AQMSMLPRLRPKEFYDLVIEVAIVRPGPIQGGAVHPYLRRRQKLEPVSYPSPELKIALER
TLGVPIFQEQVMQVAILAAGFTPGEADQLRRAMAAWKRKGGLEKYYDRIVNGMRERGYEQ
SFAEAIFEQIKGFGEYGFPESHAASFALLVYASSWLKRHEPEAFLAAMLNSQPMGFYSPS
QLVQDARRHGVIVLPVDVTVSGWDSTLETVEGRARPAVRLGLSLLRGMKDGAAERIENAR
AVRPFRSVHDLARRAQLNRHDLHVLADANALSSLAGNRREALWHSVAAVPDKDMLAVAGV
QDDTPELGAPSEAQDIVADYRSVGLTLGRHPLDLLRPVLLEQRLMPASALRNYRNGRLAR
GCGIVTVRQRPGTAKGVLFVTIEDETGNVNVIIWPSLLEKQRREALGASLLAVYGTWQCE
GEVRHLVAQRLVDMSHLLGGLQSTSRDFH