Protein Info for QEN71_RS23885 in Paraburkholderia sabiae LMG 24235

Annotation: 3-hydroxybutyrate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 265 TIGR01963: 3-hydroxybutyrate dehydrogenase" amino acids 11 to 265 (255 residues), 340.4 bits, see alignment E=2.8e-106 PF00106: adh_short" amino acids 11 to 202 (192 residues), 180.9 bits, see alignment E=3.1e-57 PF08659: KR" amino acids 14 to 179 (166 residues), 43.3 bits, see alignment E=6.1e-15 PF13561: adh_short_C2" amino acids 17 to 262 (246 residues), 166.2 bits, see alignment E=1.5e-52

Best Hits

Swiss-Prot: 39% identical to BDHA_RHIME: D-beta-hydroxybutyrate dehydrogenase (bdhA) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00019, 3-hydroxybutyrate dehydrogenase [EC: 1.1.1.30] (inferred from 99% identity to bph:Bphy_1961)

MetaCyc: 39% identical to acetoin reductase subunit (Klebsiella pneumoniae)
RXN-11032 [EC: 1.1.1.304]; 1.1.1.- [EC: 1.1.1.304]

Predicted SEED Role

"3-hydroxybutyrate dehydrogenase (EC 1.1.1.30)" in subsystem Acetyl-CoA fermentation to Butyrate (EC 1.1.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.30

Use Curated BLAST to search for 1.1.1.30 or 1.1.1.304

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (265 amino acids)

>QEN71_RS23885 3-hydroxybutyrate dehydrogenase (Paraburkholderia sabiae LMG 24235)
MSSSNESLKGKVAVVTGAASGIGKQIALTLSAAGAAVAIADLNQDGANAVAEEINKAGGK
ALGVAMDVTNEDAVNQGIDKVAAELGSVDILISNAGIQIVNPIENYSFSDWKKMQAIHVD
GAFLTTKAALKHMYKDDRGGIVIYMGSVHSHEASPLKSAYVTAKHALLGLSRVLAKEGAK
HNVRSHVVCPGFVRTPLVDKQIPEQAKELGIPEEEVVRRVMLGGTVDGIFTTVEDVAQTV
LFLSSFPTAALTGQSFIVSHGWYMQ