Protein Info for QEN71_RS23570 in Paraburkholderia sabiae LMG 24235

Annotation: M20 aminoacylase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 390 TIGR01891: amidohydrolase" amino acids 17 to 377 (361 residues), 367.7 bits, see alignment E=3.4e-114 PF01546: Peptidase_M20" amino acids 76 to 386 (311 residues), 167.2 bits, see alignment E=4.7e-53 PF07687: M20_dimer" amino acids 188 to 284 (97 residues), 48.1 bits, see alignment E=1e-16

Best Hits

Swiss-Prot: 47% identical to HIPO_CAMJE: Hippurate hydrolase (hipO) from Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)

KEGG orthology group: K01451, hippurate hydrolase [EC: 3.5.1.32] (inferred from 94% identity to bph:Bphy_1825)

MetaCyc: 41% identical to jasmonoyl-amino acid conjugate hydrolase 1 (Nicotiana attenuata)
RXN-18481 [EC: 3.5.1.127]

Predicted SEED Role

"Catalyzes the cleavage of p-aminobenzoyl-glutamate to p-aminobenzoate and glutamate, subunit A" in subsystem p-Aminobenzoyl-Glutamate Utilization

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.32

Use Curated BLAST to search for 3.5.1.127 or 3.5.1.32

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (390 amino acids)

>QEN71_RS23570 M20 aminoacylase family protein (Paraburkholderia sabiae LMG 24235)
MNTMVIPAGIAGLEEEMIALRRRIHAHPELAYEEHVTGDLVAEKLTEWGYTVTRGLGKTG
VVGQLKVGSGTRKLGLRADMDALPIHEQTGLPYASKLPGKMHACGHDGHTAMLLAAAKHL
AQERSYDGTLNLIFQPAEEGLAGAKKMIEDGLFERFPCDAVFAMHNMPGFPTGKFGFLPG
SFMASSDTVIIKVIGRGGHGAMPHKAVDAVVVCAQIVLALQTIVSRNVGPLDMAIITVGA
IHAGEAPNVIPESAEMRLSVRALKPEVRNYLETRIQEVVHAQAAVYNARAEIDYQRRYPV
LVNDVQMTQFATQIARDWVGDDGLIANMQPLTGSEDFAFMLERCAGSYLIIGNGDGEGGC
MVHNPGYDFNDDCLATGAAYWVRLAQAFLV