Protein Info for QEN71_RS23515 in Paraburkholderia sabiae LMG 24235

Annotation: histidine ammonia-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 507 TIGR01225: histidine ammonia-lyase" amino acids 1 to 497 (497 residues), 700.8 bits, see alignment E=4.4e-215 PF00221: Lyase_aromatic" amino acids 8 to 466 (459 residues), 632.5 bits, see alignment E=2e-194

Best Hits

Swiss-Prot: 92% identical to HUTH_PARPJ: Histidine ammonia-lyase (hutH) from Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN)

KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 98% identity to bph:Bphy_1814)

MetaCyc: 63% identical to histidase subunit (Pseudomonas putida)
Histidine ammonia-lyase. [EC: 4.3.1.3]

Predicted SEED Role

"Histidine ammonia-lyase (EC 4.3.1.3)" in subsystem Histidine Degradation (EC 4.3.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.3

Use Curated BLAST to search for 4.3.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (507 amino acids)

>QEN71_RS23515 histidine ammonia-lyase (Paraburkholderia sabiae LMG 24235)
MILTPGSLTLTQLRQIAREQVTLELDPASFAAIDACAQAVADIAAKGEPAYGINTGFGRL
ASTHIPHDQLELLQRNLVLSHAVGVGEPMSQPVVRLLMALKLSSLGRGHSGIRREVMEAL
IKLFNADVLPVIPVKGSVGASGDLAPLAHMSAVLLGVGEVFAKGERVAATEGLRLVGLKP
MTLQAKEGLALLNGTQASTALALYNMFAIEDLYRTGLVAGALSVDAAAGSVKPFDARIHE
LRGHQGQIDAAGSYRALLDGSAINLSHRDCGKVQDPYSLRCQPQVMGACLDQMRAAAQVL
LVEANAVSDNPLIFPDTGEVLSGGNFHAEPVAFAADNLALAASEIGALAERRIALLIDAT
LSGLPPFLVKDGGVNSGFMIAHVTAAALASENKTLAHPASVDSLPTSANQEDHVSMATFA
ARKLADIADNTANILSIELLAAAQGVDLRAPHKTSPALQKVMDTVRAEVAHYELDHYFAP
DIAAITKLVQDGTIAKHSPFSFISEQR