Protein Info for QEN71_RS23300 in Paraburkholderia sabiae LMG 24235

Annotation: MdtA/MuxA family multidrug efflux RND transporter periplasmic adaptor subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 474 transmembrane" amino acids 38 to 56 (19 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 96 to 424 (329 residues), 250.7 bits, see alignment E=9.1e-79 PF13533: Biotin_lipoyl_2" amino acids 121 to 169 (49 residues), 45 bits, see alignment 1.1e-15 PF16576: HlyD_D23" amino acids 123 to 343 (221 residues), 46.4 bits, see alignment E=4.5e-16 PF13437: HlyD_3" amino acids 232 to 333 (102 residues), 27.7 bits, see alignment E=5.7e-10

Best Hits

Swiss-Prot: 46% identical to MDTA_YERE8: Multidrug resistance protein MdtA (mdtA) from Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)

KEGG orthology group: K07799, putative multidrug efflux transporter MdtA (inferred from 88% identity to bph:Bphy_1771)

MetaCyc: 45% identical to multidrug efflux pump membrane fusion protein MdtA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-352; TRANS-RXN-353; TRANS-RXN-92

Predicted SEED Role

"Probable Co/Zn/Cd efflux system membrane fusion protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (474 amino acids)

>QEN71_RS23300 MdtA/MuxA family multidrug efflux RND transporter periplasmic adaptor subunit (Paraburkholderia sabiae LMG 24235)
MDEQKKQPESTRHPEPASPPPGREPGPGAVKRHRGRTIALIVAVVLVAGIVLVRWHPWNK
SASGASTATGASGAHAGRTGGRRGAGAGGMDQPQPVHVASATQGEMPVVLNALGTVTPLA
NVTVRTQLSGVLQTVAFQEGQMVKKGDLLAQVDPRPYQISLENAQGTLAKDQALLQTARL
DLKRYQTLLSQDSIASQQVDTQASLVKQYEGQVKADQANIDTFKLDLNYARITAPVAGRV
GLRQVDAGNYVTPGDTNGVVVITQLQPISVIFTTSEDNLPAILKQYHAGQKMSVTAYDRS
NTTSLEAGYLETLDNQIDTTTGTVKLRATFQNNGGLLFPNQFVNTRLLVNVVKNAVIVPT
SSVLNGSMGAFVYVVKPDNTVTVRKVKVGPVDGERTSIASGLEVGERVVIDGSDRLKEGA
KITIPADRPRGASGASGASGASGASAAHAASGASAALAASGARRHKHASQTSAE