Protein Info for QEN71_RS17730 in Paraburkholderia sabiae LMG 24235

Annotation: 1,4-alpha-glucan branching protein GlgB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 741 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 126 to 739 (614 residues), 894 bits, see alignment E=2.6e-273 PF02922: CBM_48" amino acids 131 to 214 (84 residues), 84.3 bits, see alignment E=9.3e-28 PF00128: Alpha-amylase" amino acids 288 to 360 (73 residues), 25.9 bits, see alignment E=1.1e-09 PF02806: Alpha-amylase_C" amino acids 643 to 740 (98 residues), 85.6 bits, see alignment E=3.6e-28

Best Hits

Swiss-Prot: 84% identical to GLGB_PARXL: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Paraburkholderia xenovorans (strain LB400)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 96% identity to bph:Bphy_4391)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (741 amino acids)

>QEN71_RS17730 1,4-alpha-glucan branching protein GlgB (Paraburkholderia sabiae LMG 24235)
MSEQHNVQLEAAGLNRVDLDALVDARHHDPFSQLGMHDTHSGPVVRVMLPNAGRVSVIAR
DDSRLLGELEQLHPGGLFAGPVSEAVPYRLRIDWHGSVQEIEDTYSFGPVLGDEPLQRVA
WGDPYAVLECLGSRPYTMDGVPGVRFAVWAPNARRVSVVGDFNSWDGRRHPMRMRHQAGV
WELFVPRIGAGTRYKYEMLTRDGHPLPLKADPCAMQTEKPPATASIVAHVDEVERYPWTD
GDWMQTRAGKQTAQSAISIYEVHAESWLRIAEEGHRGLNWSELADRLIPYAKEMGFTHIE
FMPVAEHPFGGSWGYQPLSQFAPSARFGTPEQFAGFVNRAHEAGLGVILDWVPAHFPNDS
HGLVEFDGTPLYEHADPREGYHQDWNTMIYNLGRNEVSAFLIASALAWLKRYHVDGLRVD
AVASMLYRDYSRAAGEWVPNIYGGRENLESIAFLKRLNHEVQHMPDVHGAITIAEESTAW
PGVTARTEDGGLGFQFKWNMGWMHDTLHYMHEDPVYRQWHHHNMTFAMVYAYSEKFVLPL
SHDEVVHGKGSLLGKMPGDRWQKFANLRAYFAFMWTHPGKKLMFMGGEFGQMAEFDHDGS
PHWHLLDDDLHHGVQRLVRDLNRLYREEPALHRLDTEPGGFEWIIGDDTANSVFAWRRVD
GAGRELITVCNFTPVPRHGYRVGLPRAGRWVEVLNSDAGVYGGSNVGNGGVIYTDEISAH
GRPQSAALVLPPLGTVVLRAD