Protein Info for QEN71_RS17665 in Paraburkholderia sabiae LMG 24235

Annotation: bifunctional tetrahydrofolate synthase/dihydrofolate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 27 to 430 (404 residues), 337.1 bits, E=9.4e-105
• PF08245: Mur_ligase_M
  • amino acids 47 to 268 (222 residues), 41.4 bits, E=1.6e-14

Best Hits

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 97% identity to bph:Bphy_4378)

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17)" (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

• superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
• superpathway of tetrahydrofolate biosynthesis (9/10 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• folate polyglutamylation (4/5 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)
• superpathway of chorismate metabolism (42/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Protein Sequence (436 amino acids)

>QEN71_RS17665 bifunctional tetrahydrofolate synthase/dihydrofolate synthase (Paraburkholderia sabiae LMG 24235)
MTTFPTLDAWLTHLESAHPVGIDMGLARISQVRDALQLSFACPVITVGGTNGKGSTCAIL
ESILLRAGYSVGCHTSPHLLSFNERARINGEMATDAALLPHFEAVEKARRSLPEPVSLTY
FEFTTLAIMHLFAERGLDAVIFEVGLGGRLDAVNILDTDCAIITSIDIDHTDFLGDTRDK
IAFEKAGIFRAGKPAICADPVPPQTLIDHAEKIGADLWLFGRDFRYEAQPGNERQQWSYI
GPSMRRSALAYPALRGANQLINTTAALAGLEALRDRLPVSAQDIRLGLANVELPGRFQVL
PGKPSIVLDVGHNPHAAAVLGQNLGNMGYFPYTYAVFGSMRDKDIAGVLSHLTGEIDHWN
VTDLPTPRAASAEELDAALRDAGVTDGPDGGVKRFATPAEAFQDALKRASENDRIVVFGS
FYTVAGVMAYRKSQQH