Protein Info for QEN71_RS16030 in Paraburkholderia sabiae LMG 24235

Annotation: ribulose-bisphosphate carboxylase large subunit family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02788: RuBisCO_large_N
  • amino acids 18 to 131 (114 residues), 30.8 bits, E=3.1e-11
• PF00016: RuBisCO_large
  • amino acids 141 to 421 (281 residues), 253.5 bits, E=2.7e-79

Best Hits

Swiss-Prot: 48% identical to RBLL_BORBR: Uncharacterized ribulose bisphosphate carboxylase-like protein (BB1035) from Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50)

KEGG orthology group: K01601, ribulose-bisphosphate carboxylase large chain [EC: 4.1.1.39] (inferred from 81% identity to bxe:Bxe_B0441)

MetaCyc: 46% identical to 3-oxoisoapionate-4-phosphate transcarboxylase/hydrolase (Xanthobacter autotrophicus Py2)
RXN-20936 [EC: 3.7.1.28]

Predicted SEED Role

"similar to ribulose-1,5-bisphosphate carboxylase, Type III"

MetaCyc Pathways

• Rubisco shunt (10/10 steps found)
• Calvin-Benson-Bassham cycle (11/13 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
• ethene biosynthesis V (engineered) (19/25 steps found)
• D-apionate degradation III (RLP transcarboxylase/hydrolase) (3/3 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• oxygenic photosynthesis (12/17 steps found)
• nucleoside and nucleotide degradation (archaea) (1/10 steps found)

KEGG Metabolic Maps

• Carbon fixation in photosynthetic organisms
• Glyoxylate and dicarboxylate metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.39

Use Curated BLAST to search for 3.7.1.28 or 4.1.1.39

Protein Sequence (425 amino acids)

>QEN71_RS16030 ribulose-bisphosphate carboxylase large subunit family protein (Paraburkholderia sabiae LMG 24235)
MNARDDSIEADYLIETPLDLAKVADTMAGEQSSGTFVRVANESDELRARSRANVLRIEEL
EPAPHASLPSAWLMRQRIDGPFRRARVTLSFPIANIGANLPTLAATVAGNLYDLGEVTGM
RLLSMRVPRAYRERFDMPVHGVAGTRRLTGVQNGPMVGTIIKPNVGLSAEETAQLVKTLC
EAGIDFIKDDEVCANPLHAPLDQRVRAVMREVRAFRERTGKRVMVAFNITDDVDAMRRHA
ELVEREEGDCVMASINWCGFSAIQALRRSTPLVLHAHRNGYGMMSRDAALGIGFQAYQTL
WRLAGVDHMHVHGLAGKFAQSDAEVSESARDCSTPLAEGLDDRVLPAFSSGQWAGTVPAT
FDAIGSTDLLFMSGGGVLAHPDGPAAGVRSVRQAWDAAQSRTPLHEHAKHAPELRAALAF
FGERT