Protein Info for QEN71_RS15680 in Paraburkholderia sabiae LMG 24235

Annotation: HlyD family efflux transporter periplasmic adaptor subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 420 transmembrane" amino acids 39 to 60 (22 residues), see Phobius details PF16576: HlyD_D23" amino acids 186 to 306 (121 residues), 45.4 bits, see alignment E=9.4e-16 PF00529: CusB_dom_1" amino acids 189 to 363 (175 residues), 38.5 bits, see alignment E=1.4e-13 PF13437: HlyD_3" amino acids 235 to 314 (80 residues), 53.7 bits, see alignment E=4.5e-18

Best Hits

Swiss-Prot: 49% identical to EMRA_ECOLI: Multidrug export protein EmrA (emrA) from Escherichia coli (strain K12)

KEGG orthology group: K03543, multidrug resistance protein A (inferred from 92% identity to bph:Bphy_4021)

MetaCyc: 49% identical to multidrug efflux pump membrane fusion protein EmrA (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-363; TRANS-RXN-364; TRANS-RXN-365

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (420 amino acids)

>QEN71_RS15680 HlyD family efflux transporter periplasmic adaptor subunit (Paraburkholderia sabiae LMG 24235)
MSEIENTERETTQATQAAAPRAKPEAASAAEPNTRKRKLLLSLLGAAVVASAAAYGAYYM
TYARYHESTDDAYVSGNLVQLTPQVTGTVIAVNADDTQIVKMGDSVVKLDNADAKVALGN
AEATLGQTVRQVSSLYVNNDFYAANVAQKQSDLARAQDDLRRRQAVAGTGAVSAEDIAHA
RDTVTAAQAALDAARQQAEANRALTDRTTVAEHPNVQAAASKVRDAYLAYARNTLPAPVT
GYVAKRSVQVGQRVSPGTPLMAIVPLDGVWVDANYKESQLRNMRIGQPVTLTADVYGGKV
EYHGRVVGFSAGTGSAFATLPAQNATGNWIKVVQRLPARIQLDPKELEAHPLRIGLSMDV
DVDTRDNTGEQLGAAMNTTYRTDVFAEYGAQADAEIDRIIAQNMVPTHAEAARPLPKHAG