Protein Info for QEN71_RS14675 in Paraburkholderia sabiae LMG 24235

Annotation: gamma-glutamyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 62% identical to GGT_PSEAE: Glutathione hydrolase proenzyme (ggt) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00681, gamma-glutamyltranspeptidase [EC: 2.3.2.2] (inferred from 93% identity to bph:Bphy_3857)

Predicted SEED Role

"Gamma-glutamyltranspeptidase (EC 2.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Utilization of glutathione as a sulphur source (EC 2.3.2.2)

MetaCyc Pathways

γ-glutamyl cycle (5/6 steps found)
glutathione degradation (DUG pathway) (2/2 steps found)
leukotriene biosynthesis (2/6 steps found)
hypoglycin biosynthesis (4/14 steps found)
peptido-conjugates in tissue regeneration biosynthesis (6/17 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.2.2

Use Curated BLAST to search for 2.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (576 amino acids)

>QEN71_RS14675 gamma-glutamyltransferase (Paraburkholderia sabiae LMG 24235)
MKLIDKAKFSATALAVVGLFTTSAGFLESAPAYAKAPSAQQPVLNASAVAVADKYSADAA
EQIFKEGGNAVDAAVAIAFTLAVTYPEAGNIGGGGFMTLYVNGKPYFIDYRERAPQAATR
NMYLDDQGNVIKGKSLYGYYAAGVPGTVAGMWEAQHRFGKLKWKQVLAPAIKYARDGFEV
DAQLAKRREDAEKDFAGKTNFDTYFGNLKEGVNFKQPDLAAVLTRIANQGAREFYNGKTA
DLIASAMKGHGLITKRDLQDYRAVWRQPVQADWNGYRVITAPPPSSGGIGLVQLLKMKAD
LKSDFTNVDLNSPQYIHLISEIEKRVFADRAQYLGDPDFYKVPVAQLTDDAYIAKRASEV
NPSAPSDTKSVVAGLGMSMPEKAETTHFSVVDKWGNAVSNTYTINGYFGSGVVAEGTGIV
LNDEMDDFASKPGVPNMFGVVGSDANSIEPKKRPLSSMTPTILTKDGKVSLVIGTPGGSR
IFTSIFQVINNVYDFNMPLKDAVGAMRFHHQLLPPNTIFWEPYAPIDGELAKQVEAKGYK
LKAQDFNGDIQAIKIDGESPNAVSDPRGRGVTRVIP