Protein Info for QEN71_RS12760 in Paraburkholderia sabiae LMG 24235

Annotation: ectoine hydrolase DoeA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 406 TIGR02993: ectoine utilization protein EutD" amino acids 16 to 405 (390 residues), 763.5 bits, see alignment E=2.5e-234 PF01321: Creatinase_N" amino acids 28 to 173 (146 residues), 64.4 bits, see alignment E=1.5e-21 PF00557: Peptidase_M24" amino acids 180 to 389 (210 residues), 121.9 bits, see alignment E=3.4e-39

Best Hits

Swiss-Prot: 66% identical to DOEA_HALED: Ectoine hydrolase (doeA) from Halomonas elongata (strain ATCC 33173 / DSM 2581 / NBRC 15536 / NCIMB 2198 / 1H9)

KEGG orthology group: K01271, Xaa-Pro dipeptidase [EC: 3.4.13.9] (inferred from 93% identity to bxe:Bxe_C0063)

MetaCyc: 66% identical to ectoine hydrolase (Halomonas elongata DSM 2581)
RXN-18397 [EC: 3.5.4.44]

Predicted SEED Role

"Aminopeptidase YpdF (MP-, MA-, MS-, AP-, NP- specific)" in subsystem Protein degradation

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.4.13.9

Use Curated BLAST to search for 3.4.13.9 or 3.5.4.44

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (406 amino acids)

>QEN71_RS12760 ectoine hydrolase DoeA (Paraburkholderia sabiae LMG 24235)
MSETTQTETRNATPGVRLPFERAEYATRLAKTRKAMQAAGIELLIVSDPTNMAWLTGYDG
WSFYVHQCVLLALDGEPVWFGRGQDANGAKRTVFMEHEHIVGYPDHYVQSPVRHPMDYLA
KEVIDARGWGALRIGVEMDNYYFSAAAYRSLVANLPNAKWQDATALVNWQRAVKSPREIE
YMRVAARIVEQMHAHIVERIEPGMRKNDLVAEIYATGITGVDGYGGDYPAIVPLLPTGAD
AAAPHLTWDDTPFAKDAGTFFEIAGCFKRYHCPQSRTVYLGKPPKHFLDGERAVVEGIEA
GLAAAKPGNTCEDIANAFFAMLHKYGIEKNSRCGYPIGASYPPDWGERTMSLRPGDKTVL
EPGMTFHFMPGLWLDDWGLEITESILITETGVETLCNTPRKLFVKP