Protein Info for QEN71_RS10530 in Paraburkholderia sabiae LMG 24235

Annotation: phospholipase C, phosphocholine-specific

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 718 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details TIGR03396: phospholipase C, phosphocholine-specific" amino acids 5 to 710 (706 residues), 927.9 bits, see alignment E=3.8e-283 TIGR01409: Tat (twin-arginine translocation) pathway signal sequence" amino acids 5 to 27 (23 residues), 19.1 bits, see alignment (E = 1.2e-07) PF04185: Phosphoesterase" amino acids 48 to 435 (388 residues), 394.8 bits, see alignment E=4e-122 PF05506: PLipase_C_C" amino acids 506 to 592 (87 residues), 98.7 bits, see alignment E=3.1e-32 amino acids 616 to 697 (82 residues), 70.6 bits, see alignment E=1.8e-23

Best Hits

KEGG orthology group: K01114, phospholipase C [EC: 3.1.4.3] (inferred from 83% identity to bpy:Bphyt_7094)

Predicted SEED Role

"Phospholipase C 4 precursor (EC 3.1.4.3)" (EC 3.1.4.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.4.3

Use Curated BLAST to search for 3.1.4.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (718 amino acids)

>QEN71_RS10530 phospholipase C, phosphocholine-specific (Paraburkholderia sabiae LMG 24235)
MTSDSRRRFLKTAATSAGAAAAVTMLPESIRNALAVPANSRTGSIRDVEHIVVFMQENRS
FDHYFGHMRGVRGYNDRFPIPLPNGKPVWYQPSKADATKPVLPFHLDTQTTSAQCVGDLD
HSWYKTHGAIDAGRYDQWPANKTDMTMGYHLRSDIPFHYALADAFTVCDAYFCSLPGPTH
PNRSYLMTGTVDPTGTKGGPLLDNNDFVDGDVPPKYQLLSWTTYPERLESAGISWQIYQQ
GTDGSDPLNGNYGTNILQNFENFINAQPGSSLFQRAQTVRTIDDLKADVQANRLPQVSWL
CPPAAYSEHPKYTPAYGAEYTSQILDALTSNPEMWSKTVLFIMYDENDGFFDHIVPPQPA
TSRAQGLSTVTTDGEIHNIVNPARGGSYTADNLPYGLGPRVPMTIVSPWTKGGFVCSQVF
DHTSVIRFIESRFGVHEPNITAWRRAVCGDLTTAFDFRTPDAKMPSLPDTSNYKSMADNQ
CATQPAPTVPAVPGPFDPQESGIRFARALPYELHVNGKVDQKANSLTIEIGNTGDQGAHF
YVYSTNRTDGPWRYTVEAGKTLKDVFNLSSTNGVYQFTVYGPNGFVRNFAGNAQGQSSGN
QVNAAQSGHNRKPALPEVKTHYDVGNGNVYLKFTNHGDSLARLSVVDNAYGAHPRPVIVP
PGASIEEAWVLSASHHWYDLTVSDNDDASFQRRLAGHVETGKTSISDPAAVTPVMAAS