Protein Info for QEN71_RS07535 in Paraburkholderia sabiae LMG 24235

Annotation: aromatic ring-hydroxylating dioxygenase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00355: Rieske
  • amino acids 45 to 126 (82 residues), 74.7 bits, E=4.6e-25
• PF00848: Ring_hydroxyl_A
  • amino acids 191 to 425 (235 residues), 65.3 bits, E=7.5e-22

Best Hits

KEGG orthology group: None (inferred from 92% identity to bge:BC1002_5120)

MetaCyc: 60% identical to 2-(3-hydroxy-2-oxo-1H-indol-3-yl)acetate dioxygenase IacC subunit (Pseudomonas putida 1290)
RXN-20920

Predicted SEED Role

"Benzoate 1,2-dioxygenase alpha subunit (EC 1.14.12.10)" in subsystem Benzoate degradation (EC 1.14.12.10)

MetaCyc Pathways

• indole-3-acetate degradation I (3/3 steps found)
• benzoate degradation I (aerobic) (2/2 steps found)
• mandelate degradation to acetyl-CoA (10/18 steps found)
• superpathway of aromatic compound degradation via 3-oxoadipate (22/35 steps found)
• meta cleavage pathway of aromatic compounds (4/10 steps found)
• toluene degradation IV (aerobic) (via catechol) (4/13 steps found)
• superpathway of aerobic toluene degradation (12/30 steps found)
• superpathway of aromatic compound degradation via 2-hydroxypentadienoate (16/42 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Fluorobenzoate degradation

Isozymes

Compare fitness of predicted isozymes for: 1.14.12.10

Use Curated BLAST to search for 1.14.12.10

Protein Sequence (427 amino acids)

>QEN71_RS07535 aromatic ring-hydroxylating dioxygenase subunit alpha (Paraburkholderia sabiae LMG 24235)
MSDTTWQTIDTSALSGRVQSDRIAPSLYYDPNLFEAELERIFYKTWVWVAHESELPNPGD
FRTTTIGKQPVIVVRDKTGTVNVLQNRCRHRGATVCEEHKGNAKGFTCPYHSWSYALDGT
LRALPYGDGYEGVCEKGDLPLAKLRVGIYQGLIFASFNDEIEPLEDFLGGAKPWIDLFMK
QGAGYPIKANGEHKFKFKGNWKIQLENTTDLYHFPVVHKSWMKSIDDETAAAITSFMTSE
DAFCRSLGNGHSLAVLVPEIVDLDKDDGAPLPERFNELAATLAERHSPEEVRRIVRSLMG
VGFNLNLFPNLALSMAFFRVLRPISAEETEIRHVALAMDGGPDEANRVRLRIHEHFQGPF
GFGSPDDAEAWERVQRGSYAGPDVPILVNRGLNRETTAANGEKTAHATDETGMREAYRQW
RAMMEQA