Protein Info for QEN71_RS06005 in Paraburkholderia sabiae LMG 24235

Annotation: GTPase HflX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03156: GTP-binding protein HflX
  • amino acids 9 to 361 (353 residues), 488.3 bits, E=5.6e-151
• PF13167: GTP-bdg_N
  • amino acids 22 to 110 (89 residues), 106.1 bits, E=3.6e-34
• PF16360: GTP-bdg_M
  • amino acids 112 to 190 (79 residues), 105.6 bits, E=4.8e-34
• PF02421: FeoB_N
  • amino acids 197 to 359 (163 residues), 31.3 bits, E=4.2e-11
• PF01926: MMR_HSR1
  • amino acids 198 to 317 (120 residues), 77.1 bits, E=3.6e-25

Best Hits

Swiss-Prot: 53% identical to HFLX_ECOLI: GTPase HflX (hflX) from Escherichia coli (strain K12)

KEGG orthology group: K03665, GTP-binding protein HflX (inferred from 98% identity to bph:Bphy_1414)

MetaCyc: 53% identical to ribosome rescue factor HflX (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein HflX" in subsystem Hfl operon or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (414 amino acids)

>QEN71_RS06005 GTPase HflX (Paraburkholderia sabiae LMG 24235)
MTSTNLINAALVGIDFGKIDFEASLEELSLLAQSAGAHPAVTLTGRRSSPDAAMFVGSGK
AEELRIACEANDIDIVIFNHALAPAQQRNLERTLNRRVVDRTSLILDIFAQRARSHEGKL
QVELAQLQYLATRLVRAWTHLERQKGGIGLRGPGETQLETDRRLIGERIKMLKGRLEKLR
RQHGTQRRARARNRTMSVSLVGYTNAGKSTLFNALTKAQAYAADQLFATLDTTSRRVYLG
EEVGQIVVSDTVGFIRELPHQLVAAFRATLEETIHADLLLHVVDASSAVRLDQIDQVNDV
LREIGADTIRQVLVFNKIDAVPELAARGDAVERDEYGNISRVFLSARTGQGLDTLRAAIA
EIATAEQLPESDGLLSEGDPRAAAIYQEQRDNAGEDDRDDRKIPDTGADPFQLH