Protein Info for QEN71_RS05975 in Paraburkholderia sabiae LMG 24235

Annotation: flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 437 TIGR00612: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase" amino acids 25 to 418 (394 residues), 386.6 bits, see alignment E=5.9e-120 PF04551: GcpE" amino acids 40 to 419 (380 residues), 375.6 bits, see alignment E=1.1e-116

Best Hits

Swiss-Prot: 92% identical to ISPG_BURCJ: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG) from Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)

KEGG orthology group: K03526, (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase [EC: 1.17.7.1] (inferred from 98% identity to bph:Bphy_1420)

Predicted SEED Role

"1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (EC 1.17.7.1)" in subsystem Isoprenoid Biosynthesis (EC 1.17.7.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.17.7.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (437 amino acids)

>QEN71_RS05975 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase (Paraburkholderia sabiae LMG 24235)
MHSDQVKSSSQIVSTVPVFGGHAPRRRSHAVDVRWGGQLVTIGGDAPVRVQSMTNTDTAD
AIGTAIQIKELAQAGSELVRITVNTPEAAAAVPAVREQLDRMGVTVPLVGDFHYNGHLLL
RDYPGCAESLSKYRINPGNVGQGAKRDTQFAQMIEAAAKYDKPVRIGVNWGSLDQDLLAK
MMDENAARAEPWEAQSVMYEALIQSAIGSAERAVELGLGRDKIILSCKVSGVQDLIAVYQ
ELAKRCDFALHLGLTEAGMGSKGIVASTAALSVLLQQGIGDTIRISLTPEPGASRTGEVI
VGQEILQTMGLRSFTPMVIACPGCGRTTSTLFQELASQIQTYLRTQMPMWRDTYPGVEKM
HVAVMGCIVNGPGESKHANIGISLPGSGENPAAPVFIDGVKVKTLRGENIAQEFQQIVSD
YVERSYGPNAVRAEATT