Protein Info for QEN71_RS05385 in Paraburkholderia sabiae LMG 24235

Annotation: Zn-dependent hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01879: amidase, hydantoinase/carbamoylase family
  • amino acids 21 to 418 (398 residues), 414.9 bits, E=1.7e-128
• PF04389: Peptidase_M28
  • amino acids 75 to 142 (68 residues), 22.5 bits, E=1.2e-08
• PF01546: Peptidase_M20
  • amino acids 92 to 417 (326 residues), 101.3 bits, E=1.1e-32

Best Hits

Swiss-Prot: 57% identical to HYUC_RHIML: N-carbamoyl-L-amino-acid hydrolase (hyuC) from Rhizobium meliloti

KEGG orthology group: K06016, N-carbamoyl-L-amino-acid hydrolase [EC: 3.5.1.87] (inferred from 97% identity to bph:Bphy_1536)

MetaCyc: 57% identical to N-carbamoyl-L-amino-acid hydrolase subunit (Sinorhizobium meliloti CECT4114)
N-carbamoyl-L-amino-acid hydrolase. [EC: 3.5.1.87]

Predicted SEED Role

"Beta-ureidopropionase (EC 3.5.1.6)" in subsystem Hydantoin metabolism or L-2-amino-thiazoline-4-carboxylic acid-Lcysteine conversion or Pyrimidine utilization (EC 3.5.1.6)

MetaCyc Pathways

• thymine degradation (3/3 steps found)
• uracil degradation I (reductive) (3/3 steps found)
• superpathway of pyrimidine ribonucleosides degradation (3/5 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Pantothenate and CoA biosynthesis
• Pyrimidine metabolism
• beta-Alanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.6, 3.5.1.87

Use Curated BLAST to search for 3.5.1.6 or 3.5.1.87

Protein Sequence (425 amino acids)

>QEN71_RS05385 Zn-dependent hydrolase (Paraburkholderia sabiae LMG 24235)
MNAVSEALKQGLDTSIAVNGKRLWDSLMTMAKIGATQKGGVCRLALTDLDKEGRDLIVSW
AKDAGCTVSVDQMGNVFMRRAGTNPNALPVMTGSHADSQPTGGRFDGIYGVLGGLEVIRS
LNDRNIETEHPIEVVIWTNEEGSRFAPAMVASGVFAGVFTLDYGLSRKDVDGKTIGEELK
RIGYAGDVPCGGRPLHAAFELHIEQGPILEAENKTIGVVTDAQGQRWYEITLTGQEAHAG
PTPMPRRKDALLGAARVVDLVNRIGLDHAPLACATVGMMQVHPNSRNVIPGRVFFTVDFR
HPDDAVLAKMDAALREGVAKIAGGIGLETQLEQIFYYEPVRFDAACVKSVRAAAVRFGYS
HRDMVSGAGHDACYLSQVAPTSMVFVPCVDGISHNEIEDATPEWIEAGANVLLHAMLERA
CEPAS