Protein Info for QEN71_RS05350 in Paraburkholderia sabiae LMG 24235

Annotation: benzoate-CoA ligase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02262: benzoate-CoA ligase family
  • amino acids 22 to 528 (507 residues), 759.3 bits, E=1e-232
• PF00501: AMP-binding
  • amino acids 35 to 393 (359 residues), 276.2 bits, E=4e-86
• PF13193: AMP-binding_C
  • amino acids 443 to 520 (78 residues), 70.6 bits, E=1.7e-23

Best Hits

KEGG orthology group: K04110, benzoate-CoA ligase [EC: 6.2.1.25] (inferred from 93% identity to bph:Bphy_1543)

Predicted SEED Role

"Benzoate-CoA ligase (EC 6.2.1.25)" in subsystem Benzoate transport and degradation cluster (EC 6.2.1.25)

MetaCyc Pathways

• benzoate degradation II (aerobic and anaerobic) (1/1 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (5/9 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (4/17 steps found)
• anaerobic aromatic compound degradation (Thauera aromatica) (5/27 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.25

Protein Sequence (532 amino acids)

>QEN71_RS05350 benzoate-CoA ligase family protein (Paraburkholderia sabiae LMG 24235)
MQALLETPPGEPAPKVEAPPAQFNFASHLFRLNDTRADKAAYIDDAGVTSFAQLEDRARR
FASALRAIGVHAEERILLVMLDTAELPVAFLGALYAGVVPVVANTLLTAADYLYMLTHSH
ARAVIASGALLPAVEQAMSEAEHDGCLLIVSQPVHLDPPPSHVLNQLIDGAEPLLKPNAC
SGDDIAFWLYSSGSTGKPKGTVHTHANLYWTAELYAKPILGITERDVVFSAAKLFFAYGL
GNALTFPLSVGATAVLMAERPTPDAIFRRLVEHRPTIFYGVPTLYANMLVSPNLPPRGQV
AMRVCTSAGEALPREIGERFTAHFGCEILDGIGSTEMLHIFLSNRAGEVEYGTTGRPVPG
YEVELRDEAGHPVPDGEVGDLFIKGPSAALMYWSNREKSRATFLGEWIRSGDKYCRLPNG
CYQYAGRSDDMLKVSGQYVSPVEVEMVLVQHTAVLEAAVVGIDHGGLVKTRAFVVLKQTA
DACDALADELKSFVKGKLAPHKYPRDIVFVDDLPKTATGKIQRFKLREQLNS