Protein Info for QEN71_RS05110 in Paraburkholderia sabiae LMG 24235

Annotation: 3'-5' exonuclease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 796 PF00580: UvrD-helicase" amino acids 8 to 269 (262 residues), 298 bits, see alignment E=3.3e-92 PF13245: AAA_19" amino acids 12 to 254 (243 residues), 81.5 bits, see alignment E=2.7e-26 PF13361: UvrD_C" amino acids 274 to 650 (377 residues), 263.2 bits, see alignment E=1.8e-81 PF13538: UvrD_C_2" amino acids 590 to 646 (57 residues), 38.5 bits, see alignment 3.4e-13 PF21196: PcrA_UvrD_tudor" amino acids 747 to 790 (44 residues), 25.2 bits, see alignment 4.9e-09

Best Hits

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 98% identity to bph:Bphy_1590)

Predicted SEED Role

"ATP-dependent DNA helicase UvrD/PcrA" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (796 amino acids)

>QEN71_RS05110 3'-5' exonuclease (Paraburkholderia sabiae LMG 24235)
MPELLANLNPEQHAAVTLPNEPALILAGAGSGKTRVLITRIAWLIQQGFASPPTVLGVTF
TNKAAREMMSRLSALLPIDTRGMWIGTFHGLCNRMLRAHYRDAGLPQTFQILDTSDQLSA
IKRLMKGLNIDDEKYPAKNLQYFINNAKEQGLRPKDVDASDNFNRKFVELYEAYDQQCQR
EGVVDFPELLLRCYELLSYNPPLRAHYQARFRHILVDEFQDTNKLQYAWLKMLAGEHNAI
FAVGDDDQSIYAFRGANVGNMRDFESEFKVRNLIKLEQNYRSHGHILDAANHLIANNSRR
LGKNLRTDAGHGEPVRVYEAATDSQEAGWIVEEIRALINTGLSRSEIAVLYRSNAQSRTI
EHTLVNVGIPYRVYGGLRFFERQEVKHALAYLRLIDNPNDDTAFARVVNFPTRGIGARSI
EQLADAARLYNCSMAAAIPYVTGKAGTSLGTFANLIAKMRAETAQMSLPETVEYVVRASG
LSEFYQNEREGQERLENLQELVNAATAFVSEEGYGLDTPARSIPLRPGATSAPELVVSTD
DPGVDVLDAANPADPAQNPDTMTPLAGFLSHASLEAGDNQAQAGQEAVQLMTVHAAKGLE
FTAVFITGLEEGLFPHENSAMESDGLEEERRLMYVAITRAKERLYLSFAQSRMLHGQTRY
NIRSRFFDELPQETLKWLTPKVEAGARWGGGRSDNAAWGRDWFSRPDRQQGGGYQGGYQG
RESYATTNAPLPAFANEQRAADTGFRVGQQVFHTKFGEGTITALEGGGADAKALVKFKRH
GEKWLALAVAKLQAVE