Protein Info for QEN71_RS04400 in Paraburkholderia sabiae LMG 24235

Annotation: ATP-dependent helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 734 PF00580: UvrD-helicase" amino acids 50 to 316 (267 residues), 205 bits, see alignment E=4.7e-64 PF13245: AAA_19" amino acids 52 to 302 (251 residues), 71.4 bits, see alignment E=2.2e-23 PF13361: UvrD_C" amino acids 323 to 471 (149 residues), 88.7 bits, see alignment E=1.4e-28 amino acids 601 to 663 (63 residues), 61.4 bits, see alignment E=2.8e-20 PF13538: UvrD_C_2" amino acids 604 to 662 (59 residues), 41.1 bits, see alignment 3.2e-14

Best Hits

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 94% identity to bph:Bphy_0801)

Predicted SEED Role

"ATP-dependent DNA helicase UvrD/PcrA, proteobacterial paralog" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (734 amino acids)

>QEN71_RS04400 ATP-dependent helicase (Paraburkholderia sabiae LMG 24235)
MLSVADSPSSSPSPAPVEATSASASAAAADWLAKLNEAQREAVEYGTDDVAKPSGALLVI
AGAGSGKTNTLAHRVANLVVKGTDPRRILLLTFSRRAALEMTRRVTRIASAALGTRAALA
QGLTWSGTFHSVGARLLREYADLIGLSPTFTINDREDSADLMNLVRHELGFSAKEKRFPS
KSACFAIYSRVVNTGASLADVLNSAFPWCREWEADLRMLFAAYVDAKQKQSVLDYDDLLL
YWSHMAAEPAIAADLSGRFDHVLVDEYQDTNRLQASILLALKPDGRGLTVVGDDAQSIYS
FRGATVRNILDFPAHFDPPAKQVTLERNYRSTQPILEASNAVIDLSTERYTKNLWTDKAS
AQRPHLVTVADDADQARYIVEQVLAAREAGMKLKSQAVLFRAAHHSATLEIELTRRNIPF
VKFGGLKFLDSVHVKDVLAVLRWAENPRDRVAGFRVVQLLPGVGPATAARVLDDIAARAD
ALGSGSALDVAHGVSGCAAGALAAFAPPARTLEDWHLFVAMMASVCGRQTPWPAEFEMVR
RWYEPHLERNHEDASIRQADLVQMESIAGTYASRERFLTELTLDPPDATSDESGVPLIDE
DYLILSTIHSAKGQEWRNVFVLNGVDGCIPSDLGTGSEEEIDEERRLLYVAMTRAKEDLH
IVMPQRFYVHNQTHLGDRHVWASRTRFIPPHLLPLFDSHAWPPVPVVSAPTSAGLAAAAQ
AKIEIAAKLRKMWD