Protein Info for QEN71_RS04000 in Paraburkholderia sabiae LMG 24235

Annotation: formate dehydrogenase beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 518 transmembrane" amino acids 199 to 221 (23 residues), see Phobius details amino acids 305 to 325 (21 residues), see Phobius details PF01512: Complex1_51K" amino acids 146 to 315 (170 residues), 146.7 bits, see alignment E=5.5e-47 PF10589: NADH_4Fe-4S" amino acids 426 to 507 (82 residues), 94.9 bits, see alignment E=2.2e-31

Best Hits

KEGG orthology group: K00124, formate dehydrogenase, beta subunit [EC: 1.2.1.2] (inferred from 96% identity to bph:Bphy_0728)

Predicted SEED Role

"NAD-dependent formate dehydrogenase beta subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (518 amino acids)

>QEN71_RS04000 formate dehydrogenase beta subunit (Paraburkholderia sabiae LMG 24235)
MTRIYVPRDSSALALGADALATAIVDEAKARGIEVELVRNGSRGLLYLEPLIEVETPAGR
IGYANVEAEDIKPLFDAGFVEGNAHAKQVGVVDEIPYLKKQQRLTFARIGITDPLSTDDY
IANGGIVGLYNALAMTGDAAVEALIESGLRGRGGAAFPAGIKWRTVRAAKADQKYIVCNA
DEGDSGTFSDRLVMESDPFVLIEGMIIAGIVTGATVGHIYVRSEYPHSIATLEAAIVKAR
AAGWLGDSVLGSEHRFELFVAKGAGAYVCGEETALLESLEGKRGIVRAKPPLPALEGLFG
KPTVINNVITLATVPIIFAKGAAFYKDFGMGRSRGTLPFQIAGNVKQGGLVELAFGCTLR
ELMFDYGGGTASGRPARAVQVGGPLGTYLPESQWDIPLDYEEYAKVGAVVGHGGLVIHDD
TSNLADLAQYAMHFCALESCGKCTPCRIGSTRGVEVIQRIRNGDTSTKQVQLLRDLCDTM
VSGSLCAMGGMTPFPVVSALDHFPEDFGLVAATPKQAA