Protein Info for QEN71_RS03835 in Paraburkholderia sabiae LMG 24235

Annotation: aromatic amino acid ammonia-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00221: Lyase_aromatic
  • amino acids 31 to 478 (448 residues), 537.7 bits, E=1.2e-165

Best Hits

Swiss-Prot: 36% identical to HUTH_PHEZH: Histidine ammonia-lyase (hutH) from Phenylobacterium zucineum (strain HLK1)

KEGG orthology group: K01745, histidine ammonia-lyase [EC: 4.3.1.3] (inferred from 94% identity to bph:Bphy_0696)

Predicted SEED Role

"Putative histidine ammonia-lyase protein"

MetaCyc Pathways

• L-histidine degradation II (5/5 steps found)
• L-histidine degradation VI (7/8 steps found)
• L-histidine degradation I (3/4 steps found)
• L-histidine degradation III (4/6 steps found)

KEGG Metabolic Maps

• Histidine metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.3

Use Curated BLAST to search for 4.3.1.3

Protein Sequence (532 amino acids)

>QEN71_RS03835 aromatic amino acid ammonia-lyase (Paraburkholderia sabiae LMG 24235)
MSEHDLIDDTHAASAASVSAPRTVVIGGRRLSIEEVVAIAKGRAHVALSGDPAWRSRIQR
GADFLRRHLAAGETVYGVNTGYGDACVVDVPMELVEALPLQLTRYHGCGMGAHLDDAQTL
AVIAARLNSLAYGFSGVRPVLLERLADLVNHRVLPRIPSEGSVGASGDLTPLSYVAAALV
GERDVMFDGALRDAAGVWTQLGHAPLMLAPKEGLALMNGTAVMTGLACLAFARAEHLARV
VARLTALSTVALDGRAAHFDAMIFEAKPHAGQADAAAWIRADLEGRDDTPGHRLQDRYSI
RCAPHVIGVVVDALSWVRRDVENELNSANDNPLIDPDGERVLHGGNFYGGHIAFAMDALK
TAVANLADLMDRQLALIVDDKFNNGLPRNLTGATSARAPINHGFKAVQISSSAWTAEALK
HTMPASVFSRSTEAHNQDKVSMGTIAARDCLRVLELTEQVAAAHTLAAVQALKLRVRLND
ATLVPAPLRAFAESVASMSPFVDEDRALESDLRALTARIADCALIEGGAHHE